Results 21 to 30 of about 143,834 (286)

Light-controlled cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides [PDF]

, 2022
Cell-free expression of a gene to protein has become a vital tool applied in DNA nanodevices and synthetic cells. Antisense oligonucleotides are often used to induce gene knockdown; however, they have been underexplored for cell-free applications ...
Denis, Hartmann, Michael, Booth
core   +2 more sources

Targeting TGFβ Signaling to Address Fibrosis Using Antisense Oligonucleotides

Biomedicines, 2018
Fibrosis results from the excessive accumulation of extracellular matrix in chronically injured tissue. The fibrotic process is governed by crosstalk between many signaling pathways.
James T. March, Golnoush Golshirazi, Viktorija Cernisova, Heidi Carr, Yee Leong, Ngoc Lu-Nguyen, Linda J. Popplewell   +6 more
doaj   +1 more source

Polymerase-endonuclease amplification reaction (PEAR) for large-scale enzymatic production of antisense oligonucleotides. [PDF]

PLoS ONE, 2010
Antisense oligonucleotides targeting microRNAs or their mRNA targets prove to be powerful tools for molecular biology research and may eventually emerge as new therapeutic agents.
Xiaolong Wang, Deming Gou, Shuang-yong Xu   +2 more
doaj   +1 more source

Targeting FMN, TPP, SAM-I, and glmS Riboswitches with Chimeric Antisense Oligonucleotides for Completely Rational Antibacterial Drug Development

Antibiotics, 2023
Antimicrobial drug resistance has emerged as a significant challenge in contemporary medicine due to the proliferation of numerous bacterial strains resistant to all existing antibiotics.
Nikolet Pavlova, Martina Traykovska, Robert Penchovsky   +2 more
doaj   +1 more source

Consequences of Making the Inactive Active Through Changes in Antisense Oligonucleotide Chemistries

Frontiers in Genetics, 2019
Antisense oligonucleotides are short, single-stranded nucleic acid analogues that can interfere with pre-messenger RNA (pre-mRNA) processing and induce excision of a targeted exon from the mature transcript.
Khine Zaw, Khine Zaw, Kane Greer, Kane Greer, May Thandar Aung-Htut, May Thandar Aung-Htut, Chalermchai Mitrpant, Chalermchai Mitrpant, Rakesh N. Veedu, Rakesh N. Veedu, Sue Fletcher, Sue Fletcher, Steve D. Wilton, Steve D. Wilton   +13 more
doaj   +1 more source

Design of a nanobiosystem with remote photothermal gene silencing in Chlamydomonas reinhardtii to increase lipid accumulation and production

Microbial Cell Factories, 2023
Research development in the precise control of gene expression in plant cells is an emerging necessity that would lead to the elucidation of gene function in these biological systems.
Hossein Alishah Aratboni, Nahid Rafiei, Ashanti Concepción Uscanga-Palomeque, Itza Eloisa Luna Cruz, Roberto Parra-Saldivar, Jose Ruben Morones-Ramirez   +5 more
doaj   +1 more source

Sarm1 haploinsufficiency or low expression levels after antisense oligonucleotides delay programmed axon degeneration

Cell Reports, 2021
Summary: Activation of the pro-degenerative protein SARM1 after diverse physical and disease-relevant injuries causes programmed axon degeneration. Original studies indicate that substantially decreased SARM1 levels are required for neuroprotection ...
Stacey Anne Gould, Jonathan Gilley, Karen Ling, Paymaan Jafar-Nejad, Frank Rigo, Michael Coleman   +5 more
doaj   +1 more source

Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes [PDF]

, 1994
Encapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides.
Zelphati, Olivier, Leserman, Lee, Wagner, Ernst   +2 more
core   +1 more source

Taking control of gene expression with light-activated oligonucleotides

BioTechniques, 2007
The recent development of caged oligonucleotides that are efficiently activated by ultraviolet (UV) light creates opportunities for regulating gene expression with very high spatial and temporal resolution.
Ivan J. Dmochowski, XinJing Tang
doaj   +1 more source

Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acids Potently Reduce Ebola Virus Infection In Vitro

Molecular Therapy: Nucleic Acids, 2019
Ebola virus is the causative agent of Ebola virus disease, a severe, often fatal illness in humans. So far, there are no US Food and Drug Administration (FDA)-approved therapeutics directed against Ebola virus.
Anne Sadewasser, Erik Dietzel, Sven Michel, Michael Klüver, Markus Helfer, Tamara Thelemann, Richard Klar, Markus Eickmann, Stephan Becker, Frank Jaschinski   +9 more
doaj   +1 more source