Results 31 to 40 of about 72,368 (158)

Taking control of gene expression with light-activated oligonucleotides

BioTechniques, 2007
The recent development of caged oligonucleotides that are efficiently activated by ultraviolet (UV) light creates opportunities for regulating gene expression with very high spatial and temporal resolution.
Ivan J. Dmochowski, XinJing Tang
doaj   +1 more source

Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acids Potently Reduce Ebola Virus Infection In Vitro

Molecular Therapy: Nucleic Acids, 2019
Ebola virus is the causative agent of Ebola virus disease, a severe, often fatal illness in humans. So far, there are no US Food and Drug Administration (FDA)-approved therapeutics directed against Ebola virus.
Anne Sadewasser, Erik Dietzel, Sven Michel, Michael Klüver, Markus Helfer, Tamara Thelemann, Richard Klar, Markus Eickmann, Stephan Becker, Frank Jaschinski   +9 more
doaj   +1 more source

Structural Diversity of Sense and Antisense RNA Hexanucleotide Repeats Associated with ALS and FTLD

Molecules, 2020
The hexanucleotide expansion GGGGCC located in C9orf72 gene represents the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD).
Tim Božič, Matja Zalar, Boris Rogelj, Janez Plavec, Primož Šket   +4 more
doaj   +1 more source

A Kinetic Model Explains Why Shorter and Less Affine Enzyme-recruiting Oligonucleotides Can Be More Potent

Molecular Therapy: Nucleic Acids, 2014
Antisense oligonucleotides complementary to RNA targets promise generality and ease of drug design. The first systemically administered antisense drug was recently approved for treatment and others are in clinical development. Chemical modifications that
Lykke Pedersen, Peter H Hagedorn, Marie Wickström Lindholm, Morten Lindow   +3 more
doaj   +1 more source

Antisense Oligonucleotides: Concepts and Pharmaceutical Applications

Borneo Journal of Pharmacy, 2023
Antisense oligonucleotides are drugs whose mechanism is based on binding to RNA target sequences. For this purpose, they modify the protein expression through steric hindrance and exon omission.
Ariana Araya, David Arias, Karla Coto, María Rebeca López, Joselyn Rivera, Juan José Mora   +5 more
doaj   +1 more source

In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations

Scientific Reports, 2021
Liver and kidney uptake and antisense activity is studied for a series of Locked Nucleic Acid (LNA) oligonucleotides with fully stereo-defined, internucleoside linkages.
Henrik Frydenlund Hansen, Nanna Albaek, Bo Rode Hansen, Irene Shim, Henrik Bohr, Troels Koch   +5 more
doaj   +1 more source

Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites

Retrovirology, 2007
Background A primary concern when targeting HIV-1 RNA by means of antisense related technologies is the accessibility of the targets. Using a library selection approach to define the most accessible sites for 20-mer oligonucleotides annealing within the ...
Berkhout Ben, Wengel Jesper, Haasnoot Joost, Jakobsen Martin R, Kjems Jørgen   +4 more
doaj   +1 more source

Transport Oligonucleotides—A Novel System for Intracellular Delivery of Antisense Therapeutics

Molecules, 2020
Biological activity of antisense oligonucleotides (asON), especially those with a neutral backbone, is often attenuated by poor cellular accumulation. In the present proof-of-concept study, we propose a novel delivery system for asONs which implies the ...
Oleg V. Markov, Anton V. Filatov, Maxim S. Kupryushkin, Ivan V. Chernikov, Olga A. Patutina, Anton A. Strunov, Elena L. Chernolovskaya, Valentin V. Vlassov, Dmitrii V. Pyshnyi, Marina A. Zenkova   +9 more
doaj   +1 more source

Investigating discovery strategies and pharmacological properties of stereodefined phosphorodithioate LNA gapmers

Molecular Therapy: Nucleic Acids, 2022
The introduction of sulfur into the phosphate linkage of chemically synthesized oligonucleotides creates the stereocenters on phosphorus atoms. Researchers have valued the nature of backbone stereochemistry and early on investigated drug properties for ...
Jörg Duschmalé, Adrian Schäublin, Erik Funder, Steffen Schmidt, Łukasz J. Kiełpiński, Helle Nymark, Klaus Jensen, Troels Koch, Martina Duschmalé, Erich Koller, Marianne Ravn Møller, Simone Schadt, Christophe Husser, Andreas Brink, Sabine Sewing, Tanja Minz, Jesper Wengel, Konrad Bleicher, Meiling Li   +18 more
doaj   +1 more source

Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNA/LNA-Based Apolipoprotein B Antisense Inhibitors

Journal of Nucleic Acids, 2012
The duplex stability with target mRNA and the gene silencing potential of a novel bridged nucleic acid analogue are described. The analogue, 2′,4′-BNANC antisense oligonucleotides (AONs) ranging from 10- to 20-nt-long, targeted apolipoprotein B.
Tsuyoshi Yamamoto, Hidenori Yasuhara, Fumito Wada, Mariko Harada-Shiba, Takeshi Imanishi, Satoshi Obika   +5 more
doaj   +1 more source