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Cryopreservation of human oocytes

Human Fertility, 2001
Cryopreservation is a well established technique by which preimplantation stage embryos can be stored for later use. However, it would be preferable to cryopreserve unfertilized oocytes to overcome the problems related to the ethical and legal status of the embryo.
G, Coticchio   +3 more
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Cryopreservation of oocytes and embryos

Theriogenology, 2014
Two hundred years have passed since the first description of supercooled water by Gey-Lussac to the recently high survival rates of embryo and oocytes after vitrification. This review discusses important milestones that have made vitrification the method of choice for oocytes and embryos cryopreservation.
openaire   +2 more sources

Oocytes cryopreservation: State of art

Reproductive Toxicology, 2006
In the present review article we sought to analyze, on the basis of a systematic review, the indications, rationale of oocytes cryopreservation, as well as the techniques that improved the aforementioned procedure in order to higher the pregnancy rate in women undergoing that procedure.
AMBROSINI, GUIDO   +8 more
openaire   +5 more sources

Cryopreservation of embryos and oocytes

Current Opinion in Obstetrics and Gynecology, 1993
Widespread incorporation of human embryo cryopreservation into IVF programs may reduce the risk of multiple gestation and severe ovarian hyperstimulation syndrome as well as contributing to an overall increase in pregnancy rates. Slow cooling techniques appropriate to the stage of embryo development are most commonly employed.
T, Gelety, E, Surrey
openaire   +2 more sources

History of oocyte cryopreservation

Reproductive BioMedicine Online, 2011
The potential advantages of being able to cryopreserve oocytes have been apparent for many decades. Technical difficulties associated with the unique properties of the mammalian oocyte initially retarded rapid development in this area but recent advances have overcome many of the problems.
openaire   +2 more sources

Cryopreservation of Mammalian Oocytes

2007
Two methods for the cryopreservation of mammalian oocytes are described. One method uses a relatively low concentration of the cryoprotectant propanediol plus sucrose and requires controlled-rate cooling equipment to achieve a slow cooling rate. Such a method has produced live births from cryopreserved human oocytes. The second method described employs
Sharon J, Paynter, Barry J, Fuller
openaire   +2 more sources

Cryopreservation of oocytes and embryos

Current Opinion in Obstetrics and Gynecology, 1991
In humans, embryo cryopreservation is now a widespread routine procedure, both reliable and flexible. It can successfully be applied to excess embryos, arising from in vitro fertilization, at any developmental stage. The role of gonadotropin-releasing hormone analogues in embryo freezing has given rise to much controversy, whereas in oocyte donation ...
openaire   +2 more sources

Cryopreservation of oocytes

2013
Introduction Oocyte cryopreservation is a major issue in human reproductive medicine. The need to cryopreserve oocytes rather than embryos is not limited to countries with restrictive legislation. In fact, all the major clinical applications rely on fertility preservation for both medical and non-medical reasons [1–4] and for oocyte donation programs
Alan Trounson, Laura Rienzi
openaire   +1 more source

Technical aspects of oocyte cryopreservation

Molecular and Cellular Endocrinology, 2000
Since the successful development in the mouse, the oocyte cryopreservation has been applied with varying success to a number of different species including the human. The recently reported successes in terms of pregnancies obtained by human oocyte cryopreservation are encouraging. Several studies typically reported different rates of survival (20-80%),
R, Fabbri   +9 more
openaire   +2 more sources

Cryopreservation of Mature and Immature Oocytes

Clinical Obstetrics & Gynecology, 2010
The aim of this chapter is to evaluate the current situation concerning oocyte freezing. Clinical outcome using slow cooling and vitrification was assessed in the literature and in our clinic to evaluate possible differences using either of the protocols.
Andrea, Borini, Veronica, Bianchi
openaire   +2 more sources

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