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Cryopreservation of human oocytes*

Human Reproduction, 1987
Three different methods were used for freezing human excess oocytes (320 frozen, 205 thawed) in our IVF programme and the results of these methods were compared. A high fertilization rate (75%) could be achieved after thawing, using 1,2 propanediol as a cryoprotectant.
S, Al-Hasani   +5 more
openaire   +2 more sources

Cryopreservation of human oocytes

Human Fertility, 2001
Cryopreservation is a well established technique by which preimplantation stage embryos can be stored for later use. However, it would be preferable to cryopreserve unfertilized oocytes to overcome the problems related to the ethical and legal status of the embryo.
G, Coticchio   +3 more
openaire   +2 more sources

Oocytes cryopreservation: State of art

Reproductive Toxicology, 2006
In the present review article we sought to analyze, on the basis of a systematic review, the indications, rationale of oocytes cryopreservation, as well as the techniques that improved the aforementioned procedure in order to higher the pregnancy rate in women undergoing that procedure.
AMBROSINI, GUIDO   +8 more
openaire   +5 more sources

Cryopreservation of Mammalian Oocytes

2014
Two methods for the laboratory-focused cryopreservation of mammalian oocytes are described, based on work with murine oocytes. One method uses a relatively low concentration of the cryoprotectant propanediol plus sucrose and requires controlled rate cooling equipment to achieve a slow cooling rate.
Victoria, Keros, Barry J, Fuller
openaire   +2 more sources

History of oocyte cryopreservation

Reproductive BioMedicine Online, 2011
The potential advantages of being able to cryopreserve oocytes have been apparent for many decades. Technical difficulties associated with the unique properties of the mammalian oocyte initially retarded rapid development in this area but recent advances have overcome many of the problems.
openaire   +2 more sources

Cryopreservation of Mammalian Oocytes

2007
Two methods for the cryopreservation of mammalian oocytes are described. One method uses a relatively low concentration of the cryoprotectant propanediol plus sucrose and requires controlled-rate cooling equipment to achieve a slow cooling rate. Such a method has produced live births from cryopreserved human oocytes. The second method described employs
Sharon J, Paynter, Barry J, Fuller
openaire   +2 more sources

Fresh versus cryopreserved oocyte donation

Current Opinion in Endocrinology, Diabetes & Obesity, 2016
Purpose of review Utilization of cryopreserved instead of fresh donor oocytes has rapidly increased in recent years. Whether treatment outcomes are comparable has, however, remained controversial. Recent findings More than 24% of initiated oocyte donation cycles in the USA during ...
Vitaly A, Kushnir, Norbert, Gleicher
openaire   +2 more sources

Oocyte cryopreservation in oncological patients

European Journal of Obstetrics & Gynecology and Reproductive Biology, 2004
The use of chemotherapy and radiotherapy in oncological patients may reduce their reproductive potential. Sperm cryopreservation has been already used in men affected by neoplastic disease. Oocyte cryopreservation might be an important solution for these patients at risk of losing ovarian function.
PORCU, ELEONORA   +5 more
openaire   +3 more sources

Oocyte Cryopreservation Technique

2017
Cryopreservation of human oocytes can be performed by both slow freezing and vitrification. In 1986, the first report of a pregnancy from frozen-thawed oocytes was obtained [1]. Since this report, many efforts were made to improve the efficiency of the cryopreservation protocols, both for slow-freezing and vitrification.
Neelke De Munck   +2 more
openaire   +1 more source

Technical aspects of oocyte cryopreservation

Molecular and Cellular Endocrinology, 2000
Since the successful development in the mouse, the oocyte cryopreservation has been applied with varying success to a number of different species including the human. The recently reported successes in terms of pregnancies obtained by human oocyte cryopreservation are encouraging. Several studies typically reported different rates of survival (20-80%),
R, Fabbri   +9 more
openaire   +2 more sources

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