Advanced Science, EarlyView.SIRT5 desuccinylates and stabilizes RAC2, activating CSF1R‐dependent signaling to drive monocyte differentiation into M0 macrophages and their polarization toward pro‐inflammatory M1 phenotypes in CTX‐induced premature ovarian insufficiency. Inhibiting the SIRT5‐RAC2 axis attenuates inflammation, reduces granulosa cell apoptosis, and preserves ...
Wenjing TanTai +15 more
wiley +1 more source
Single‐Cell Profiling: Any Scale, Any Size, All at Once
Advanced Science, EarlyView.Traditional single‐cell technologies are constrained by cellular dimension and throughput scale, in addition to its inability preserve spatial information. With recent innovations, these technologies now allow high throughput, large‐sized cell, and multi‐modal profiling of patient samples, overcoming these limitations.
Denise Goh +4 more
wiley +1 more source
Reproductive BioMedicine Online, 2002 This paper describes the technical approach to treatment of age-related oocyte aneuploidy. Although one solution can be oocyte/embryo selection, another is represented by the nuclear transplantation procedure. The efficiency of nuclear transplantation into immature oocytes is described as a way of generating embryos, and the possibility that viable ...
Gianpiero D, Palermo +2 more
openaire +2 more sources
Generating golden Syrian hamsters with conditional alleles via zygote microinjection of CRISPR/Cas9
Animal Models and Experimental Medicine, EarlyView.We established the first conditional knockout (cKO) model in the golden Syrian hamster by CRISPR/Cas9‐mediated genome editing. Cas9 protein, two sgRNAs, and a donor plasmid carrying loxP‐flanked exon 2 of the ApoF gene were microinjected into one‐cell embryos. The floxed allele was efficiently generated (up to 27%) and transmitted through the germline.
Wei Chen +16 more
wiley +1 more source
Hydroxycarboxylic Acid Receptor 2 Mediates β‐hydroxybutyrate's Antiseizure Effect in Mice
Annals of Neurology, EarlyView.A Objective The ketogenic diet, a high‐fat, low‐carbohydrate regimen, is often used to treat drug‐resistant seizures and is being studied for Alzheimer's disease and other neuropsychiatric disorders. However, its mechanism of action remains unclear. β‐hydroxybutyrate, a primary circulating ketone body produced by the ketogenic diet, may mediate its ...
Soudabeh Naderi +10 more
wiley +1 more source
The MinK Potassium Channel Exists in Functional and Nonfunctional Forms When Expressed in the Plasma Membrane of \u3cem\u3eXenopus\u3c/em\u3e Oocytes [PDF]
, 1994 The minK protein induces a slowly activating voltage-dependent potassium current when expressed in Xenopus oocytes. In order to measure the levels of minK protein in the plasma membrane, we have modified the minK gene by inserting a 9 amino acid epitope ...
Blumenthal, Edward M +1 more
core +1 more source
Putrescine delays postovulatory aging of mouse oocytes by upregulating PDK4 expression and improving mitochondrial activity [PDF]
, 2018 Wendan Xu +8 more
openalex +1 more source
Variability in spawning frequency and reproductive development of the narrow-barred Spanish mackerel (Scomberomorus commerson) along the west coast of Australia [PDF]
, 2004 The narrow-barred Spanish mackerel (Scomberomorus commerson) is widespread throughout the Indo-West Pacific region. This study describes the reproductive biology of S.
Gaughan, Daniel J. +3 more
core
Glucocorticoid exposure affects female fertility by exerting its effect on the uterus but not on the oocyte: lessons from a hypercortisolism mouse model [PDF]
, 2018 Qiannan Li +7 more
openalex +1 more source
The Current Status of Semen and Oocytes Cryopreservation
, 2023 M. L. Mphaphathi +2 more
openalex +1 more source