Results 141 to 150 of about 135,056 (273)
Advances in large DNA fragment assembly for microbial cell factory engineering
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang +5 more
wiley +1 more source
Defining the SOS operon of coliphage 186
We have sequenced the LexA-controlled operon of coliphage 186 that carries the tum gene, whose product is necessary for UV induction of the 186 prophage. The operon consists of orf95 and orf97, and we have identified orf95 as the tum gene.
Brumby, A. +7 more
core +1 more source
This study evaluates the impact of aguamiel syrup on a synbiotic fermented dairy product, focusing on antihypertensive peptide release and Lacticaseibacillus rhamnosus GG survival. While metabolic parameters remained unchanged, aguamiel syrup enhanced bioactive peptide production and antihypertensive activity (45.3%), suggesting its potential as a ...
Olvera‐Rosales Laura Berenice +6 more
wiley +1 more source
Miniaturized Droplet‐Based Adaptation of the Ames Test for High‐Throughput Mutagenicity Assessment
ABSTRACT This work presents the first demonstration of a tube‐based droplet microfluidic implementation of the Ames test, bridging single‐droplet resolution with regulatory genotoxicity testing. The Ames test is a cornerstone assay for detecting mutagenicity, but conventional plate‐ and well‐based formats suffer from high reagent consumption, low ...
Jialan Cao +3 more
wiley +1 more source
Comprehensive Characterization of fucAO Operon Activation in Escherichia coli [PDF]
Wildtype Escherichia coli cells cannot grow on L-1,2-propanediol, as the fucAO operon within the fucose (fuc) regulon is thought to be silent in the absence of L-fucose.
Juan Velo +11 more
core +1 more source
Dry‐season pollutant concentration drives microbial restructuring in Bangladesh's Shitalakshya River, shifting communities from Myroides to stress‐tolerant genera while enriching metal resistance genes and efflux pumps, revealing non‐antibiotic co‐selection as a key driver of antimicrobial resistance in urban waters.
Muhammad Ehteshamul Haque +3 more
wiley +1 more source
Histidine accumulation promotes tetracycline‐induced persister formation in V. splendidus. It enhances membrane potential and upregulating the efflux pump gene, thereby reducing antibiotic susceptibility. ABSTRACT Vibrio splendidus is an opportunistic pathogen widely distributed in marine environments, animal tissues and seabed sediments. It can infect
Yanan Li +4 more
wiley +1 more source
A Transcriptional Switch for Recombinant Protein Expression in Seed Plastids
Plant Biotechnology Journal, EarlyView.
Malihe Mirzaee +3 more
wiley +1 more source
Abstract Catecholamines are classically viewed as neuronal transmitters and adrenal hormones; however, accumulating evidence demonstrates that sources other than nerve fibres and adrenal medulla play a fundamental role in local organ regulation. Physiological paradoxes, such as preserved organ function after denervation or transplantation, challenge a ...
Mariana G. de Oliveira +3 more
wiley +1 more source
ERF transcription factor StPti5 is a regulator of endophyte community maintenance in potato
Summary We have recently identified an ethylene response factor, StPti5, as a susceptibility factor that negatively regulates immune responses to diverse pathogens. Here, we investigated the role of StPti5 in the processes involved in the colonization of potato with beneficial organisms.
Tjaša Lukan +18 more
wiley +1 more source

