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Regulation of the lactose operon in vitro
Molecular and General Genetics MGG, 1970Using DNA of phage ϕ80p lac carrying lac-operon (i+o+z+y+), the transcription of the lac-operon was studied in vitro. The lac-specific mRNA having a molecular size of 28S was detected by hybridizing messenger RNA (mRNA) from induced cells (diploid for the lac-operon) to the ϕ80p lac DNA. The hybridization of lac-specific mRNA to ϕ80p lac DNA was almost
Y, Iida +3 more
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Journal of Molecular Biology, 2013
The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
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The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
openaire +2 more sources
Improved BioCyc Operon Prediction: Revisiting the Operon Prediction Problem
Abstract Introduction Operon prediction is a valuable component of microbial-genome annotation because operon organization can yield inferences about gene function, and because knowledge of operon structure can aid the interpretation of gene expression data.Peter E. Midford +2 more
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Designing Biological Circuits: Synthetic Biology Within the Operon Model and Beyond
Annual Review of Biochemistry, 2021Max A English +2 more
exaly
2000
Abstract The culmination of the work on gene action and its regulation occurred in 1961 with the formulation of the Operon Model by Jacob and Monod. This combined all the steps of protein synthesis, so carefully assembled since 1953, with the regulatory mechanisms described in Chapters 12 and 13, in a complete picture.
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Abstract The culmination of the work on gene action and its regulation occurred in 1961 with the formulation of the Operon Model by Jacob and Monod. This combined all the steps of protein synthesis, so carefully assembled since 1953, with the regulatory mechanisms described in Chapters 12 and 13, in a complete picture.
openaire +1 more source