Results 41 to 50 of about 116,936 (212)

An inducible fusaric acid tripartite efflux pump contributes to the fusaric acid resistance in Stenotrophomonas maltophilia. [PDF]

PLoS ONE, 2012
BACKGROUND: Fusaric acid (5-butylpicolinic acid), a mycotoxin, is noxious to some microorganisms. Stenotrophomonas maltophilia displays an intrinsic resistance to fusaric acid.
Rouh-Mei Hu, Sih-Ting Liao, Chiang-Ching Huang, Yi-Wei Huang, Tsuey-Ching Yang   +4 more
doaj   +1 more source

Genetic Impacts on the Structure and Mechanics of Cellulose Made by Bacteria

Advanced Science, EarlyView.
A genetic mutation in a protease of a cellulose‐overproducing evolved bacterium significantly affects the proteome, structure, and mechanical properties of bio‐fabricated cellulose pellicles. By overexpressing synthase proteins that build the cellulose‐forming biological machinery of the cell, the evolved bacterium generates dense, stiff, and strong ...
Julie M. Laurent, Mathias Steinacher, Anton Kan, Maximilian Ritter, Mario Leutert, Siiri Bienz, David Häberlin, Naresh Kumar, André R. Studart   +8 more
wiley   +1 more source

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

mBio
We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion ...
Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin   +5 more
doaj   +1 more source

Use of a Role-Playing Activity To Increase Student Understanding of Bacterial Gene Regulation

Journal of Microbiology & Biology Education, 2023
Undergraduate students often struggle to understand the basics of bacterial gene regulation, a key concept in microbiology. They find it hard to visualize the architecture of a bacterial operon or how the gene, RNA, and protein components interact with ...
Vinaya Sampath
doaj   +1 more source

Engineering von Transmembran‐Alkan‐Monooxygenasen zur Verbesserung des Schlüsselschritts in der Biosynthese des Polymervorläufers Tulipalin A

Angewandte Chemie, EarlyView.
Ein Biosyntheseweg für den Polymervorläufer Tulipalin A ausgehend von Isoprenylacetat wird hier präsentiert. Die Alkan‐Monooxygenase AlkB katalysiert die chemo‐ und regioselektive terminale Hydroxylierung von Isoprenylacetat und die Aktivität konnte durch rationelles Engineering des Membranenzyms weiter gesteigert werden. Die vollständige Oxidation zur
Andrea Nigl, Veronica Delsoglio, Lucija Sovic, Marina Grgić, Lenny Malihan‐Yap, Kamela Myrtollari, Jelena Spasic, Margit Winkler, Gustav Oberdorfer, Andreas Taden, Iva Anić, Robert Kourist   +11 more
wiley   +1 more source

Engineering of Transmembrane Alkane Monooxygenases to Improve a Key Reaction Step in the Synthesis of Polymer Precursor Tulipalin A

Angewandte Chemie International Edition, EarlyView.
A biosynthesis route for the polymer precursor tulipalin A starting from isoprenyl acetate is proposed. The alkane monooxygenase AlkB catalyzes the chemoselective terminal hydroxylation of isoprenyl acetate. Rational enzyme engineering further increased the activity of the membrane enzyme.
Andrea Nigl, Veronica Delsoglio, Lucija Sovic, Marina Grgić, Lenny Malihan‐Yap, Kamela Myrtollari, Jelena Spasic, Margit Winkler, Gustav Oberdorfer, Andreas Taden, Iva Anić, Robert Kourist   +11 more
wiley   +1 more source

Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda

Veterinary Research, 2021
Edwardsiella tarda is a facultative intracellular pathogen in humans and animals. The Gram-negative bacterium is widely considered a potentially important bacterial pathogen.
Chunmei Du, Xiaoping Huo, Hanjie Gu, Dongmei Wu, Yonghua Hu   +4 more
doaj   +1 more source

Emergence of Staphylococcus aureus Resistance to Antimicrobial Peptides Nisin, NZ2114 and Bacitracin Involves Multiple Phenotypic Changes

Animal Research and One Health, EarlyView.
The AMP‐induced mutant Staphylococcus aureus strain nzR showed increased resistance, five gene mutations, and decreased surface anion levels. AMPs affect the NDH‐2 protein encoded by ndh gene inhibiting the growth of S. aureus. The deletion of the ndh gene promotes the development of AMPs resistance.
Lingyu Xiao, Xingyuan Ma, Ping Tian, Lianbin Li, Zafir Muhammad, Xiaokun Wang, Xuemei Xue, Jinhe Xiao, Liangjun Zheng, F. A. El‐Gohary, Xin Zhao, Junning Pu, Huping Xue   +12 more
wiley   +1 more source

Periodontal microbiology and microbial etiology of periodontal diseases: Historical concepts and contemporary perspectives

Periodontology 2000, EarlyView., 2023
Abstract This narrative review summarizes the collective knowledge on periodontal microbiology, through a historical timeline that highlights the European contribution in the global field. The etiological concepts on periodontal disease culminate to the ecological plaque hypothesis and its dysbiosis‐centered interpretation.
Georgios N. Belibasakis, Daniel Belstrøm, Sigrun Eick, Ulvi K. Gursoy, Anders Johansson, Eija Könönen   +5 more
wiley   +1 more source

Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions

Microbial Cell Factories, 2006
Background In an effort to identify alternate recombinant gene expression systems in Pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antABC) and ...
Lee Vincent D, Resnick Sol M, Haney Keith L, Shao Ying, Thomas Tracey C, Retallack Diane M, Squires Charles H   +6 more
doaj   +1 more source