Results 131 to 140 of about 3,058,814 (268)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Design and implementation of an electro-optical backplane with pluggable in-plane connectors

, 2010
The design, implementation and characterisation of an electro-optical backplane and an active pluggable in-plane optical connector technology is presented. The connection architecture adopted allows line cards to be mated to and unmated from a passive
Offrein, B.J., Selviah, D.R., Horst, F., Hopkins, K., Pitwon, R.C.A., Gmür, M., Wang, K., Baghsiahi, H., Dangel, R., Halter, M.   +9 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Experimental verification of the limits of optical channel intensity reciprocity

, 2012
Optical data links through the atmosphere suffer from turbulence-induced signal scintillation. In a coaxially-symmetric bidirectional link scenario, the variations of the axial intensities at both ends are correlated.
Cowley, William, Perlot, Nicolas, Grant, Ken, Giggenbach, D., Giggenbach, Dirk, Perlot, N., Cowley, W.G., Grant, K.   +7 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Eight state optical flip-flop based on coupled unidirectional ring lasers with optical feedback

, 2006
An eight-state optical flip-flop based on coupled unidirectional ring lasers with optical feedback is demonstrated. The flip-flop exhibits low power operation since it contains only one gain medium.
Z. Li, Ju, H, Zhang, S., D. Lenstra, S. Zhang, Li, Z., H. Ju, Liu, Y., Zhang, S Shaoxian, Ju, H., Liu, Y Yong, H.J.S. Dorren, Lenstra, D.; id_orcid, Lenstra, D Daan, Li, Z Zhonggui, Lenstra, D., Khoe, GD Giok-Djan, Khoe, G.D., Y. Liu, Dorren, HJS Harm, G.D. Khoe, Dorren, H.J.S.   +21 more
core   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Tissue surface as the reference arm in Fourier domain optical coherence tomography

, 2012
We present a simple method applicable to common-path Fourier domain optical coherence tomography (OCT) in which the tissue surface is used as the reference arm.
Giardini, Mario Ettore, Krstajić, Nikola; id_orcid, Brown, C Tom A, Krstajic, Nikola, Brown, C.T.A., Dholakia, Kishan, Giardini, Mario Ettore; id_orcid, Brown, Christian Tom A.   +7 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source