Results 91 to 100 of about 38,693 (276)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Enzyme-free optical DNA mapping of the human genome using competitive binding [PDF]

, 2019
Optical DNA mapping (ODM) allows visualization of long-range sequence information along single DNA molecules. The data can for example be used for detecting long range structural variations, for aiding DNA sequence assembly of complex genomes and for ...
Ebenstein, Y.,, Kk, Sriram,, Ebenstein, Yuval,, Andersson, John, Ambjornsson, Tobias,, Ebenstein, Y., Dvirnas, Albertas, Johansson, Pegah,, Kesarimangalam, Sriram, Müller, Vilhelm, Ebenstein, Yuval, Müller, Vilhelm,, M\ufcller, Vilhelm, Andersson, John,, Dvirnas, Albertas,, Singh, Vandana,, Kk, Sriram, Johansson, Pegah, Westerlund, Fredrik, Kesarimangalam, Sriram,, Westerlund, Fredrik,, Singh, Vandana, Ambjörnsson, Tobias   +22 more
core   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Bioinformatic genome analysis of the necrotrophic wheat-pathogenic fungus Phaeosphaeria nodorum and related Dothideomycete fungi

, 2011
Phaeosphaeria nodorum (anamorph: Stagonospora nodorum) is the causal agent of Stagonospora nodorum blotch (SNB, syn. glume blotch) in wheat. P. nodorum is estimated to cause up to 31% wheat yield loss worldwide.
Hane, James
core  

genome

, 1995
Optical mapping approaches are widely preferred and applied in different branches of genomic studies because of their accuracy, low cost, and high efficiency. In the current study, a sequence orientation of the Fusarium oxysporum f. sp. melongenae (FOMG)
TANYOLAÇ, MUHAMMED BAHATTİN, Muhammed Bahattin Tanyolac, Altinok, HACER HANDAN, Kılıç, Ayten, Altinok, Hacer H., Duygu Ates, Hacer H. Altinok, ATEŞ, DUYGU   +7 more
core   +1 more source

Value of MRI Outcomes for Preventive and Early‐Stage Trials in Spinocerebellar Ataxias 1 and 3

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To examine the value of MRI outcomes as endpoints for preventive and early‐stage trials of two polyglutamine spinocerebellar ataxias (SCAs). Methods A cohort of 100 participants (23 SCA1, 63 SCA3, median Scale for the Assessment and Rating of Ataxia (SARA) score = 5, 42% preataxic, and 14 gene‐negative controls) was scanned at 3T up ...
Thiago J. R. Rezende, Emilien Petit, Young Woo Park, Sophie Tezenas du Montcel, James M. Joers, Jonathan M. DuBois, H. Moore Arnold, Michal Povazan, Ipek Özdemir, Guita Banan, Romain Valabregue, Philipp Ehses, Jennifer Faber, Chiadi U. Onyike, Peter B. Barker, Jeremy D. Schmahmann, Eva‐Maria Ratai, Sub H. Subramony, Thomas H. Mareci, Khalaf O. Bushara, Henry Paulson, Thomas Klockgether, Alexandra Durr, Tetsuo Ashizawa, Christophe Lenglet, Gülin Öz, READISCA Investigators   +26 more
wiley   +1 more source

BiSCoT: Improving large eukaryotic genome assemblies with optical maps

PeerJ, 2019
ABSTRACT Motivation Long read sequencing and Bionano Genomics optical maps are two techniques that, when used together, make it possible to reconstruct entire chromosome or chromosome arms structure. However, the existing tools are often too conservative and organization of contigs into scaffolds is ...
Istace, Benjamin, Belser, Caroline, Aury, Jean-Marc   +2 more
openaire   +4 more sources

Super-Resolution Genome Mapping in Silicon Nanochannels

, 2016
Optical genome mapping in nanochannels is a powerful genetic analysis method, complementary to deoxyribonucleic acid (DNA) sequencing. The method is based on detecting a pattern of fluorescent labels attached along individual DNA molecules.
Purohit Prashant K. Purohit, Westerlund Fredrik, Green Ori, Eisenberg Eli, Jeffet Jonathan, Nilsson Adam N., Su Tianxiang, Weinhold Elmar, Shabat Doron, Grunwald Assaf, Kobo Asaf, Ambjörnsson Tobias, Ebenstein Yuval   +12 more
core   +2 more sources

Complementarity of Long‐Reads and Optical Mapping in Parkinson's Disease for Structural Variants

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Long‐read sequencing and optical genome mapping technologies have the ability to detect large and complex structural variants. This has led to the discovery of novel pathogenic variants in neurodegenerative movement disorders. Thus, we aimed to systematically compare the SV detection capabilities of OGM and ONT in Parkinson's disease.
André Fienemann, Theresa Lüth, Susen Schaake, Carolin Gabbert, Marius Möller, Hauke Busch, Katja Lohmann, Jonas A. Gustafson, Danny E. Miller, Kensuke Daida, Manabu Funayama, Nobutaka Hattori, Samia Ben Sassi, Faycel Hentati, Matthew J. Farrer, Kristian K. Ullrich, Christine Klein, Joanne Trinh   +17 more
wiley   +1 more source