Results 71 to 80 of about 38,693 (276)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Optical mapping as a routine tool for bacterial genome sequence finishing-0

, 2011
Copyright information:Taken from "Optical mapping as a routine tool for bacterial genome sequence finishing"http://www.biomedcentral.com/1471-2164/8/321BMC Genomics 2007;8():321-321.Published online 14 Sep 2007PMCID:PMC2045679.ent, red regions indicate ...
Heidi Goodrich-Blair (81292), Phil Latreille (81281), Stacie Norton (81282), John Henkhaus (81284), Brad Barbazuk (81286), Zijin Du (81289), Brad Goodner (81291), Sophie Gaudriault (54349), Steven Slater (81293), Steve Forst (81290), Creg Darby (81288), Nancy Miller (81285), Barry S Goldman (81283), Helge B Bode (81287)   +13 more
core   +1 more source

Optical genome mapping identifies a homozygous deletion in the non-coding region of the SCN9A gene in individuals from the same family with congenital insensitivity to pain

Frontiers in Genetics
We report an index patient with complete insensitivity to pain and a history of painless fractures, joint hypermobility, and behavioral problems. The index patient descends from a family with notable cases among his maternal relatives, including his aunt
Aïcha Boughalem, Viorica Ciorna-Monferrato, Natacha Sloboda, Amélie Guegan, François Page, Sophie Zimmer, Marion Benazra, Pascale Kleinfinger, Laurence Lohmann, Mylène Valduga, Aline Receveur, Fernando Martin, Detlef Trost   +12 more
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Optical mapping as a routine tool for bacterial genome sequence finishing-1

, 2011
Copyright information:Taken from "Optical mapping as a routine tool for bacterial genome sequence finishing"http://www.biomedcentral.com/1471-2164/8/321BMC Genomics 2007;8():321-321.Published online 14 Sep 2007PMCID:PMC2045679.ent, red regions indicate ...
Heidi Goodrich-Blair (81292), Phil Latreille (81281), Stacie Norton (81282), John Henkhaus (81284), Brad Barbazuk (81286), Zijin Du (81289), Brad Goodner (81291), Sophie Gaudriault (54349), Steven Slater (81293), Steve Forst (81290), Creg Darby (81288), Nancy Miller (81285), Barry S Goldman (81283), Helge B Bode (81287)   +13 more
core   +1 more source

Optical Genome Mapping for Applications in Repeat Expansion Disorders [PDF]

Current Protocols
AbstractShort tandem repeat (STR) expansions are associated with more than 60 genetic disorders. The size and stability of these expansions correlate with the severity and age of onset of the disease. Therefore, being able to accurately detect the absolute length of STRs is important.
van der Sanden, B., Neveling, K., Pang, A.W.C., Shukor, S., Gallagher, M.D., Burke, S.L., Kamsteeg, E.J., Hastie, A., Hoischen, A., Hoischen, A.   +9 more
openaire   +3 more sources

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Identification of complex and cryptic chromosomal rearrangements by optical genome mapping

Molecular Cytogenetics, 2023
Background Optical genome mapping (OGM) has developed into a highly promising method for detecting structural variants (SVs) in human genomes. Complex chromosomal rearrangements (CCRs) and cryptic translocations are rare events that are considered ...
Shanshan Shi, Peizhi Huang, Ruiling Yan, Ruiman Li   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus)

, 2013
We report the ∼2.66-Gb genome sequence of a female Yunnan black goat. The sequence was obtained by combining short-read sequencing data and optical mapping data from a high-throughput whole-genome mapping instrument.
Yang, Shuang, Jing Chen, Cockett, Noelle, Bian, Chao, Xiaoyong Du, Yaping Zhang, Bin Zhu, James Womack, Nianqing Xiao, Min Xie, Hou, Yong, Chao Bian, Bertrand Servin, Thomas Faraut, Wenguang Zhang, Jie Liang, Nie, Wenhui, Yuxiang Li, Shen, Yongyi, Jiang, Yu, Du, Xiaoyong, James Kijas, Jinquan Li, Li, Jinquan, Liang, Jie, Jun Wang, Yang Dong, Jinhuan Wang, Noelle Cockett, Zhao, Ruoping, Shuang Yang, Deacon Sweeney, Wenliang Wang, Xiao, Nianqing, Zhu, Bin, Moore, Rich, Xin Liu, Wenhui Nie, Zhang, Yaping, Wang, Jinhuan, Sayre, Brian, Peng Zeng, Tosser-Klopp, Gwenola, Zhang, Guojie, Xu, Xun, Sweeney, Deacon, Wang, Wen, Pan, Shengkai, Servin, Bertrand, Ruoping Zhao, Brian Sayre, Chen, Wenbin, Zhao, Shuhong, Shengkai Pan, Kijas, James, Yu Jiang, Gwenola Tosser-Klopp, Guojie Zhang, Yongyi Shen, Xie, Min, Xun Xu, Faraut, Thomas, Shuhong Zhao, Dong, Yang, Wang, Wenliang, Wang, Jun, Yong Hou, Wenbin Chen, Zeng, Peng, Li, Yuxiang, Zhang, Wenguang, Womack, James, Liu, Xin, Rich Moore, Wen Wang, Chen, Jing   +75 more
core   +1 more source