Results 51 to 60 of about 99,235 (279)

De novo assembly of the cattle reference genome with single-molecule sequencing. [PDF]

, 2020
BackgroundMajor advances in selection progress for cattle have been made following the introduction of genomic tools over the past 10-12 years. These tools depend upon the Bos taurus reference genome (UMD3.1.1), which was created using now-outdated ...
Bickhart, Derek M, Cole, John B, Couldrey, Christine, Dreischer, Christian, Elsik, Christine G, Ghurye, Jay, Hagen, Darren E, Hall, Richard, Hammond, John A, Hoffman, Jinna, Koren, Sergey, Li, Wenli, Liu, George, Low, Wai Y, McDaneld, Tara G, McKay, Stephanie D, Medrano, Juan F, Murdoch, Brenda M, Nandolo, Wilson, Phillippy, Adam M, Rhie, Arang, Rosen, Benjamin D, Rowan, Troy N, Schnabel, Robert D, Schroeder, Steven G, Schultheiss, Sebastian J, Schwartz, John C, Smith, Timothy PL, Snelling, Warren M, Thibaud-Nissen, Françoise, Tseng, Elizabeth, Van Tassell, Curtis P, Zimin, Aleksey   +32 more
core  

Optical Genome Mapping ( OGM) : Looking beyond karyotyping

Asian Journal of Medical Sciences
Optical genome mapping (OGM) is an advanced technology that delivers a distinct view of genome structure. It presents numerous benefits compared to conventional sequencing techniques, as well as karyotyping in identifying large-scale structural ...
Ruby Dhar , Arun Kumar , Subhradip Karmakar   +2 more
doaj   +1 more source

Optical mapping of neuronal activity during seizures in zebrafish [PDF]

, 2017
Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs.
Fornetto, C, M\ufcllenbroich, M. C, Mannaioni, G, Marchetto, Giulia, Masi, A, Pavone, F. S, Resta, F, Tiso, Natascia, Turrini, L, Vanzi, F., Vettori, Andrea   +10 more
core   +3 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

MethodsX, 2018
Next-generation genome mapping through nanochannels (Bionano optical mapping) of plant genomes brings genome assemblies to the ‘nearly-finished’ level for reliable and detailed gene annotations and assessment of structural variations.
Paola Gaiero, Hana Šimková, Jan Vrána, Federico F. Santiñaque, Beatriz López-Carro, Gustavo A. Folle, José van de Belt, Sander A. Peters, Jaroslav Doležel, Hans de Jong   +9 more
doaj   +1 more source

Utility of Optical Genome Mapping in Repeat Disorders

Clinical Genetics
ABSTRACT Genomic repeat sequences are patterns of nucleic acids that exist in multiple copies throughout the genome. More than 60 Mendelian disorders are caused by the expansion or contraction of these repeats. Various specific methods for determining tandem repeat variations have been developed.
Mehmet Burak Mutlu, Taner Karakaya, Hamide Betül Gerik Çelebi, Fahrettin Duymuş, Serhat Seyhan, Sanem Yılmaz, Uluç Yiş, Tahir Atik, Mehmet Fatih Yetkin, Hakan Gümüş   +9 more
openaire   +6 more sources

Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer

Molecular Oncology, EarlyView.
COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann, Eduardo García‐Galea, Alice O'Farrell, Zak Kinsella, Maxime Meylan, Florent Petitprez, Ingrid Arijs, Tom Venken, Hari Ps, Adrian Lärkeryd, Ian Miller, Janick Selves, Nadja Meindl‐Beinker, Fiorella Ruiz‐Pace, Elena Élez, Raquel Comas‐Navarro, Frank Lincoln, Dirk Fey, Gift Nyamundanda, Aoife Nolan, Joern Lewin, Raquel Perez‐Lopez, Jonathan Briody, Kathleen Bennett, Walter Kolch, David Matallanas, Alexander Kel, Enrique Arenas, Joaquín Arribas, Bart Ghesquière, Josep Tabernero, Julie Meilleroux, Deborah McNamara, Ray McDermott, Marvin Lim, Mary O'Reilly, Brian Bird, Lisa Stack, Lucia Moloney, Patrick Morris, Keith Egan, Maciej Milewski, Lars Scheuer, Joachim Behringer, Georg Bolz, Ramon Salazar, Cristina Santos, Andrea Ruiz, Orla Casey, Verena Murphy, Matthias Ebert, Livio Trusolino, Diether Lambrechts, Anguraj Sadanandam, Catherine Sautès‐Fridman, Jochen Prehn, Paolo Nuciforo, Jacques Fieschi, Florence Monville, Darran O'Connor, Wolf Fridman, Annette Byrne   +61 more
wiley   +1 more source

Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum [PDF]

Applied and Environmental Microbiology, 2005
ABSTRACT Rhodospirillum rubrum is a phototrophic purple nonsulfur bacterium known for its unique and well-studied nitrogen fixation and carbon monoxide oxidation systems and as a source of hydrogen and biodegradable plastic production.
Reslewic, Susan, Zhou, Shiguo, Place, Mike, Zhang, Yaoping, Briska, Adam, Goldstein, Steve, Churas, Chris, Runnheim, Rod, Forrest, Dan, Lim, Alex, Lapidus, Alla, Han, Cliff S., Roberts, Gary P., Schwartz, David C.   +13 more
openaire   +4 more sources

Somatic mutational landscape in von Hippel–Lindau familial hemangioblastoma

Molecular Oncology, EarlyView.
The causes of central nervous system (CNS) hemangioblastoma in Von Hippel–Lindau (vHL) disease are unclear. We used Whole Exome Sequencing (WES) on familial hemangioblastoma to investigate events that underlie tumor development. Our findings suggest that VHL loss creates a permissive environment for tumor formation, while additional alterations ...
Maja Dembic, Anne Nørremølle, Lilian Bomme Ousager, Lars van Brakel Andersen, Marie Louise Mølgaard Binderup, Mads Thomassen   +5 more
wiley   +1 more source

Optical Genome Mapping Reveals Genomic Alterations upon Gene Editing in hiPSCs: Implications for Neural Tissue Differentiation and Brain Organoid Research

Cells
Genome editing, notably CRISPR (cluster regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9), has revolutionized genetic engineering allowing for precise targeted modifications.
Lucia Gallego Villarejo, Wanda M. Gerding, Lisa Bachmann, Luzie H. I. Hardt, Stefan Bormann, Huu Phuc Nguyen, Thorsten Müller   +6 more
doaj   +1 more source