Results 81 to 90 of about 38,693 (276)

Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome [PDF]

, 2018
Background: Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome.
Taylor, J., Korol, A, Frenkel, Z, Pasam, R., Taylor, J, Pfeifer, M., Abraham Korol, Câmara Ferreira, Francisco, Raj Pasam, Rigault, P, Isdale, D., Kerrie Forrest, Choulet, Frédéric, Juhasz, Angela, Tinning, Matthew, Dimech, Adam, Baumann, Ute, Bernard, A., Pasam, R, Isdale, D, Taylor, Jen, Watson-Haigh, N, Korol, Abraham, Abrouk, Michael, Dangqun Cui, Keeble-Gagnère, G, Tinning, M., Paul Eckermann, Koo, D.H., Šimková, H, Isdale, Don, Nyström-Persson, Johan, Ru, Z., Choulet, F., Huang, BE, Safar, J., B. Emma Huang, Zeev Frenkel, Toegelová, H, Nolin, Marc-Alexandre, Cui, D, Juhasz, A, Šimková, Hana, Cui, Dangqun, Iwgsc, ., Baumann, U., Konkin, David, Watson-Haigh, N., Jen Taylor, Marc-Alexandre Nolin, Frenkel, Z., Cavanagh, C., Baumann, U, Jan Šafář, Matthew Tinning, Konkin, D, Forrest, K, Luo, M.-C., Darrier, B., Nolin, M.-A., Philippe Rigault, Hana Šimková, Rigault, Philippe, Ute Baumann, Don Isdale, Tibbits, J., Forrest, Kerrie, Benoît Darrier, Sharpe, A, Pasam, Raj, Rochfort, S, Zhengang Ru, Câmara, Francisco, Keeble-Gagnère, Gabriel, Câmara, F, Hayden, M, Dimech, A, Bernard, Aurélien, Šafář, Jan, Korol, A., Koo, Dal-Hoe, Doležel, J, Luo, M.C., Fleury, Delphine, Hayden, M., Appels, R, Andrew Sharpe, Fleury, D., Matthias Pfeifer, Eckermann, P, Huang, B., Nolin, M.A., Ming-Cheng Luo, Sourdille, P., Simone Rochfort, Keeble-Gagnère, G., Konkin, D., Eckermann, P., Frédéric Choulet, Rochfort, Simone, Nyström-Persson, J., Josquin Tibbits, Šimková, H., Pierre Sourdille, Ru, Z, Luo, Ming-Cheng, Forrest, K., Tibbits, Josquin, Abrouk, M, Sourdille, Pierre, Toegelová, H., Cavanagh, Colin, Michael Abrouk, Bernard, A, Dimech, A., Jaroslav Doležel, Hayden, Matthew, Francisco Câmara, Boisvert, S, Sourdille, P, Nyström-Persson, J, Aurélien Bernard, Cui, D., Šafář, J, Rochfort, S., Choulet, F, Matthew Hayden, Appels, R., Darrier, B, Koo, D.-H., David Konkin, Nathan Watson-Haigh, et al., Frenkel, Zeev, Luo, MC, Ru, Zhengang, Sébastien Boisvert, Boisvert, S., IWGSC, Tibbits, J, Rigault, P., Tinning, M, Sharpe, Andrew, Appels, Rudi, Juhász, A., Darrier, Benoît, Abrouk, M., Watson-Haigh, Nathan, Dal-Hoe Koo, Toegelová, Helena, Huang, B.E., Doležel, J., Pasam, R.K., Huang, B. Emma, Gabriel Keeble-Gagnère, Adam Dimech, Cavanagh, C, Helena Toegelová, Delphine Fleury, Sharpe, A., Câmara, F., Boisvert, Sébastien, Pfeifer, M, Koo, DH, Eckermann, Paul, Colin Cavanagh, Huang, B, Johan Nyström-Persson, Nolin, MA, Doležel, Jaroslav, Rudi Appels, Pfeifer, Matthias, Angela Juhasz, Fleury, D   +173 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Genetic and functional characterization of inherited complex chromosomal rearrangements in a family with multisystem anomalies

Genetics in Medicine Open
Purpose: Complex chromosomal rearrangements (CCRs) are rare structural variants involving 3 or more chromosomal breakpoints. Most de novo-reported CCRs pose challenges for diagnosis and management.
He Fang, Stephen M. Eacker, Yu Wu, Whitney Neufeld-Kaiser, Mercy Laurino, Siobán Keel, Marshall S. Horwitz, Yajuan J. Liu   +7 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Genetic variation in the chicken genome: insights in selection

, 2011
The chicken currently provides more than a quarter of the meat and nearly all eggs produced worldwide. For future improvements in production traits and animal welfare as well as to address future consumer demands it is necessary to understand the ...
Elferink, M.G.
core  

Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association

BMC Genomics, 2012
Background The genome of Mycobacterium avium subspecies paratuberculosis (MAP) is remarkably homogeneous among the genomes of bovine, human and wildlife isolates.
Bannantine John P, Wu Chia-wei, Hsu Chungyi, Zhou Shiguo, Schwartz David C, Bayles Darrell O, Paustian Michael L, Alt David P, Sreevatsan Srinand, Kapur Vivek, Talaat Adel M   +10 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Developing a High-Quality Linkage Map for the Atlantic Killifish Fundulus heteroclitus

G3: Genes, Genomes, Genetics, 2019
Killifish (Fundulus heteroclitus) are widely distributed among different aquatic environments where they demonstrate an impressive range of highly-plastic and locally adaptive phenotypes. High-throughput sequencing has begun to unravel the mechanisms and
Jeffrey T. Miller, Noah M. Reid, Diane E. Nacci, Andrew Whitehead   +3 more
doaj   +1 more source

Optical genome mapping enables accurate testing of large repeat expansions. [PDF]

Genome Res
Short tandem repeats (STRs) are common variations in human genomes that frequently expand or contract, causing genetic disorders, mainly when expanded.
van der Sanden B, Neveling K, Shukor S, Gallagher MD, Lee J, Burke SL, Pennings M, van Beek R, Oorsprong M, Kater-Baats E, Kamping E, Tieleman AA, Voermans NC, Scheffer IE, Gecz J, Corbett MA, Vissers LELM, Pang AWC, Hastie A, Kamsteeg EJ, Hoischen A.   +20 more
europepmc   +2 more sources