Results 161 to 170 of about 4,090,293 (192)

Testing for Facioscapulohumeral Muscular Dystrophy with Optical Genome Mapping

Current Protocols, 2023
AbstractThe introduction of optical genome mapping has improved time constraints and a lack of specificity from previous methodologies when performing genome‐wide analyses of samples. Optical genome mapping allows for the detection of structural variations, aberrations, and functionality traits from a single stained molecule of DNA.
Pratik, Koppikar, Suresh, Shenoy, Naga, Guruju, Madhuri, Hegde   +3 more
openaire   +2 more sources

Optical Nano-mapping and Analysis of Plant Genomes

2016
Application of optical mapping based on BioNano Genomics Irys(®) technology ( http://www.bionanogenomics.com/ ) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations.
Ming-Cheng, Luo, Karin R, Deal, Armond, Murray, Tingting, Zhu, Alex R, Hastie, Will, Stedman, Henry, Sadowski, Michael, Saghbini   +7 more
openaire   +2 more sources

Optical mapping of DNA: Single‐molecule‐based methods for mapping genomes

Biopolymers, 2011
AbstractThe technologies associated with DNA sequencing are rapidly evolving. Indeed, single‐molecule DNA sequencing strategies are cheaper and faster than ever before. Despite this progress, every sequencing platform to date relies on reading the genome in small, abstract fragments, typically of less than 1000 bases in length.
Robert K, Neely, Jochem, Deen, Johan, Hofkens   +2 more
openaire   +2 more sources

Optical PCR: Genomic analysis by long-range PCR and optical mapping

Mammalian Genome, 1999
Optical mapping is an approach for the rapid, automated, non-electrophoretic construction of ordered restriction maps of DNA from ensembles of single molecules. Previously, we used optical mapping to make high-resolution maps of large insert clones such as bacterial artificial chromosomes (BAC) and large genomic DNA molecules.
J, Skiadas, C, Aston, A, Samad, T S, Anantharaman, B, Mishra, D C, Schwartz   +5 more
openaire   +2 more sources

Identification and Characterization of Chromothripsis by Optical Genome Mapping

Optical genome mapping (OGM) is a robust method for identifying and characterizing chromothripsis. While conventional short-read sequencing techniques encounter challenges in detecting chromothripsis due to limitations in read length and difficulties in analyzing repetitive sequences, OGM leverages the visualization of long DNA molecules to overcome ...
Pascal, Chambon, Mathilde, Quibeuf, Anne Marie, Guerrot   +2 more
openaire   +2 more sources

A shotgun optical map of the entire Plasmodium falciparum genome

Nature Genetics, 1999
The unicellular parasite Plasmodium falciparum is the cause of human malaria, resulting in 1.7-2.5 million deaths each year. To develop new means to treat or prevent malaria, the Malaria Genome Consortium was formed to sequence and annotate the entire 24.6-Mb genome.
Z, Lai, J, Jing, C, Aston, V, Clarke, J, Apodaca, E T, Dimalanta, D J, Carucci, M J, Gardner, B, Mishra, T S, Anantharaman, S, Paxia, S L, Hoffman, J, Craig Venter, E J, Huff, D C, Schwartz   +14 more
openaire   +2 more sources

Genomics via optical mapping. III: Contiging genomic DNA.

Proceedings. International Conference on Intelligent Systems for Molecular Biology, 2000
In this paper, we describe our algorithmic approach to constructing an alignment of (contiging) a set of restriction maps created from the images of individual genomic (uncloned) DNA molecules digested by restriction enzymes. Generally, these DNA segments are sized in the range of 1-4 Mb.
Thomas S. Anantharaman, Bud Mishra, David C. Schwartz   +2 more
openaire   +2 more sources

Cytogenetics and the Revolution of Optical Genome Mapping in the Diagnosis of Diseases

Discovery Medicine
The study of chromosomal shape, characteristics, and behavior in somatic cell division (mitosis) during growth and development and in germ cell division (meiosis) during reproduction is known as cytogenetics. Many techniques can be used for cytogenetics, including fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), multicolor FISH (M ...
Osama M, Al-Amer, Yahya, Khubrani
openaire   +2 more sources

Mapping the genome one molecule at a time — optical mapping

Nature, 1995
Akhtar H. Samad, Wei Wen Cai, Xinghua Hu, Benjamin Irvin, Junping Jing, Jason Reed, Xun Meng, John Huang, Edward Huff, Brett Porter, Alex Shenkar, Thomas Anantharaman, Bhubaneswar Mishra, Virginia Clarke, Eileen Dimalanta, Joanne Edington, Catharina Hiort, Roderic Rabbah, John Skiada, David C. Schwartz   +19 more
openaire   +2 more sources

Optical Genome Mapping: A Machine-Based Platform in Cytogenomics

Optical genome mapping (OGM) has generated excitement following decades of research and development. Now, commercially available technical platforms have been used to compare various other cytogenetic and cytogenomic technologies, including karyotype, microarrays, and DNA sequencing, with impressive results.
Jing Christine, Ye, Guilin, Tang
openaire   +2 more sources