Results 251 to 260 of about 98,363 (283)

Optical mapping of DNA: Single‐molecule‐based methods for mapping genomes

Biopolymers, 2011
AbstractThe technologies associated with DNA sequencing are rapidly evolving. Indeed, single‐molecule DNA sequencing strategies are cheaper and faster than ever before. Despite this progress, every sequencing platform to date relies on reading the genome in small, abstract fragments, typically of less than 1000 bases in length.
Robert K, Neely, Jochem, Deen, Johan, Hofkens   +2 more
openaire   +2 more sources

Optical PCR: Genomic analysis by long-range PCR and optical mapping

Mammalian Genome, 1999
Optical mapping is an approach for the rapid, automated, non-electrophoretic construction of ordered restriction maps of DNA from ensembles of single molecules. Previously, we used optical mapping to make high-resolution maps of large insert clones such as bacterial artificial chromosomes (BAC) and large genomic DNA molecules.
J, Skiadas, C, Aston, A, Samad, T S, Anantharaman, B, Mishra, D C, Schwartz   +5 more
openaire   +2 more sources

Optical Nano-mapping and Analysis of Plant Genomes

2016
Application of optical mapping based on BioNano Genomics Irys(®) technology ( http://www.bionanogenomics.com/ ) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations.
Ming-Cheng, Luo, Karin R, Deal, Armond, Murray, Tingting, Zhu, Alex R, Hastie, Will, Stedman, Henry, Sadowski, Michael, Saghbini   +7 more
openaire   +2 more sources

Genomics via optical mapping. III: Contiging genomic DNA.

Proceedings. International Conference on Intelligent Systems for Molecular Biology, 2000
In this paper, we describe our algorithmic approach to constructing an alignment of (contiging) a set of restriction maps created from the images of individual genomic (uncloned) DNA molecules digested by restriction enzymes. Generally, these DNA segments are sized in the range of 1-4 Mb.
T, Anantharaman, B, Mishra, D, Schwartz
openaire   +1 more source

Deciphering Genomic Complexity of Multiple Myeloma Using Optimized Optical Genome Mapping

The Journal of Molecular Diagnostics
The genomic evaluation of multiple myeloma in routine diagnostics involves isolating plasma cells expressing CD138, usually followed by fluorescence in situ hybridization analyses. However, cell sorting often yields a limited number of cells, restricting the number of probes that can be used and limiting the analysis to a few markers required for ...
Hélène Guermouche, Pauline Roynard, Francesca Servoli, Valentin Lestringant, Benoît Quilichini, Christine Terré, Sabine Defasque, Catherine Roche-Lestienne, Dominique Penther, Agnès Daudignon   +9 more
openaire   +2 more sources

Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans

Science, 1999
A whole-genome restriction map of Deinococcus radiodurans , a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis.
J, Lin, R, Qi, C, Aston, J, Jing, T S, Anantharaman, B, Mishra, O, White, M J, Daly, K W, Minton, J C, Venter, D C, Schwartz   +10 more
openaire   +2 more sources

Genomic Mapping of Kjer Dominant Optic Atrophy

Archives of Ophthalmology, 1997
Kjer DOMINANToptic atrophy (DOA) is a rare primary degeneration of the retinal ganglion cell. In this issue of theArchives, 2 articles describing the genetic analysis of single, large, affected families confirm the initial genomic mapping of this disease to chromosome 3q27-3q28 and refine the localization of the gene to a 4 to 7 centimorgan region.1 ...
openaire   +1 more source

Optical Genome Mapping: A Machine-Based Platform in Cytogenomics

Optical genome mapping (OGM) has generated excitement following decades of research and development. Now, commercially available technical platforms have been used to compare various other cytogenetic and cytogenomic technologies, including karyotype, microarrays, and DNA sequencing, with impressive results.
Jing Christine, Ye, Guilin, Tang
openaire   +2 more sources

Mapping the genome one molecule at a time — optical mapping

Nature, 1995
Akhtar H. Samad, Wei Wen Cai, Xinghua Hu, Benjamin Irvin, Junping Jing, Jason Reed, Xun Meng, John Huang, Edward Huff, Brett Porter, Alex Shenkar, Thomas Anantharaman, Bhubaneswar Mishra, Virginia Clarke, Eileen Dimalanta, Joanne Edington, Catharina Hiort, Roderic Rabbah, John Skiada, David C. Schwartz   +19 more
openaire   +2 more sources

Correction: Optical Genome Mapping for Oncology Applications

Current Protocols, 2023
Nikhil Shri Sahajpal, Ashis K. Mondal, Alex Hastie, Alka Chaubey, Ravindra Kolhe   +4 more
openaire   +1 more source