Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A Novel Optical Instrument for Measuring Mass Concentration and Particle Size in Real Time. [PDF]
Sensors (Basel), 2023 Zhang J, Zhang Z, Hou L, Zhou W.
europepmc +1 more source
Challenges in the first all-sky optical SETI [PDF]
, 2006 The Harvard/Planetary Society all-sky optical SETI marked a significant increase in search capability and instrument complexity. It is the first all-sky optical search, the first optical search with multiple sky pixels (there are 512 in the all-sky ...
Alan Sliski +8 more
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Design and Development of a Bimodal Optical Instrument for Simultaneous Vibrational Spectroscopy Measurements. [PDF]
Int J Mol Sci, 2022 Arévalo LA +4 more
europepmc +1 more source
Mid-Infrared Circumstellar Shell Sources Discovered with Spitzer: An Obscured Population of Massive Stars? [PDF]
, 2010 We have discovered a large number of circular and elliptical shells around luminous central sources at 24 μm with the MIPS instrument on board the Spitzer Space Telescope.
Hoard, D. W. +2 more
core
Microscale application of column theory for high resolution force and displacement sensing
, 2004 We present the design, fabrication and experimental validation of a novel device that exploits the amplification of displacement and attenuation of structural stiffness in the post-buckling deformation of slender columns to obtain pico-Newton force and ...
A. V. Desai +3 more
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Optical and mechanical design of the extreme AO coronagraphic instrument MagAO-X
, 2018 Here we review the current optical mechanical design of MagAO-X. The project is post-PDR and has finished the design phase. The design presented here is the baseline to which all the optics and mechanics have been fabricated.
Close, Laird M. +11 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source