Results 31 to 40 of about 61,825 (218)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Light-field based 3D optical tweezers [PDF]

EPJ Web of Conferences
Optical tweezers are designed to trap nano- and micro-scale particles. Once trapped, it is possible to move the particles but this requires complex mechanical adjustments to the optical system.
Le Priol Etienne, Sasaki Atsushi, Beaucamp Anthony   +2 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Direct production of fermionic superfluids in a cavity-enhanced optical dipole trap

SciPost Physics
We present the production of quantum degenerate, superfluid gases of $^6$Li through direct evaporative cooling in a cavity-enhanced optical dipole trap.
Tabea N. C. Bühler, Timo Zwettler, Gaia S. Bolognini, Aurélien H. Fabre, Victor Y. Helson, Giulia Del Pace, Jean-Philippe Brantut
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Hybrid Paul-optical trap with large optical access for levitated optomechanics

Physical Review Research
We present a hybrid trapping platform that allows us to levitate a charged nanoparticle in high vacuum using either optical fields, radio-frequency fields, or a combination thereof.
Eric Bonvin, Louisiane Devaud, Massimiliano Rossi, Andrei Militaru, Lorenzo Dania, Dmitry S. Bykov, Markus Teller, Tracy E. Northup, Lukas Novotny, Martin Frimmer   +9 more
doaj   +1 more source

The trapping and detection of single atoms using a spherical mirror

New Journal of Physics, 2012
We fabricate a miniature spherical mirror for tightly focusing an optical dipole trap for neutral atoms. The mirror formation process is modelled to predict the dimensions for particular fabrication parameters.
Arpan Roy, Andrew Bah Shen Jing, Murray D Barrett   +2 more
doaj   +1 more source

Peculiarities of focusing circularly and radially polarized super-Gaussian beams using ring gratings with varying relief height

Компьютерная оптика, 2022
The focusing features of a super-Gaussian beam, as well as Laguerre-super-Gaussian (1,0) modes with radial and circular polarizations on ring gratings (direct and inverse) with a variable height of individual relief rings were investigated in this paper.
D.A. Savelyev
doaj   +1 more source