Results 51 to 60 of about 73,961 (314)
A programmable optical angle clamp for rotary molecular motors
, 2007 Optical tweezers are widely used for experimental investigation of linear molecular motors. The rates and force dependence of steps in the mechanochemical cycle of linear motors have been probed giving detailed insight into motor mechanisms. With similar
Berry, RM +13 more
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optical Binding Phenomena: Observations and Mechanisms [PDF]
, 2009 Novel results on the optical binding of optically-trapped micro-particles are presented. A sophisticated Mie scattering model is developed, capable of performing time-evolution simulations of a multi-particle system. This is used to analyse and interpret
TAYLOR, JONATHAN,MIDGLEY
core
Design of microcavity resonators for single-atom detection [PDF]
, 2007 Whispering gallery modes of a microdisk resonator are useful for the optical detection of single rubidium and cesium atoms near the surface of a substrate. Light is coupled into two high-Q whispering-gallery modes of the disk which can provide attractive
Rosenblit, Michael +4 more
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Optical Trapping of Absorbing Particles [PDF]
, 1998 Radiation pressure forces in a focussed laser beam can be used to trap microscopic absorbing particles against a substrate. Calculations based on momentum transfer considerations show that stable trapping occurs before the beam waist, and that trapping is more effective with doughnut beams.
Rubinsztein-Dunlop, H. +3 more
openaire +4 more sources
Light-field based 3D optical tweezers [PDF]
EPJ Web of ConferencesOptical tweezers are designed to trap nano- and micro-scale particles. Once trapped, it is possible to move the particles but this requires complex mechanical adjustments to the optical system.
Le Priol Etienne +2 more
doaj +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Practical axial optical trapping [PDF]
Review of Scientific Instruments, 2012 We describe a new method for calibrating optical trapping measurements in which tension is applied in the direction of the laser beam to a molecule tethered between a surface and an optically trapped bead. Specifically, we present a generally-applicable procedure for converting from the measured scattering intensity and the measured stage displacement ...
A H, Mack +3 more
openaire +2 more sources
An optically guided atomic fountain [PDF]
, 1999 This thesis describes the development of a laser-cooling experiment aimed at efficient transfer of cold atoms over a short distance, for loading into a conservative atom trap.
Davies, Hilary Jane, Davies, H.J.
core