Results 91 to 100 of about 238 (195)

Detection Method for Installation Effect of Switch Machine Movable/Static Contact Groups Based on Key Point Recognition

Chengshi guidao jiaotong yanjiu
Objective Traditional methods for detecting the installation effect of SmMSC (switch machine movable/static contact) groups are slow, inaccurate, and susceptible to human errors.
DAI Yangjing
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

On the “Tantawy technique” and other methods for analyzing fractional Fokker–Plank type equations

Journal of Low Frequency Noise, Vibration and Active Control
The family of Fokker–Planck (FP) equations has been widely used in various physical applications, especially optical physics. It has proven effective in understanding numerous nonlinear phenomena observed in optical fiber.
Aljawhara H Almuqrin, CGL Tiofack, DV Douanla, A Mohamadou, Weaam Alhejaili, Sherif ME Ismaeel, Samir A El-Tantawy   +6 more
doaj   +1 more source

Comparative analysis of the withdrawal of thin-film flow of a third-grade fluid under the influence of MHD using two homotopy based techniques

Kuwait Journal of Science
The purpose of this work is to examine the thin film flow of a third grade fluid having appropriate boundary conditions using two homotopy based techniques, that is, the optimal homotopy asymptotic method with DJ polynomial (OHAM-DJ) and the asymptotic ...
Azzh Saad Alshehry, Musaad S. Aldhabani, Alamgeer Khan, Ahmed Shafee   +3 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Strategic Fleet Planning Under Carbon Tax and Fuel Price Uncertainty: An Integrated Stochastic Model for Fleet Deployment and Speed Optimization

Mathematics
This paper presents a two-stage stochastic programming model for the joint optimization of fleet deployment and sailing speed in liner shipping under fuel price volatility and carbon tax uncertainty.
Weilin Sun, Ying Yang, Shuaian Wang
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Long-Term Optimal Scheduling of Hydro-Photovoltaic Hybrid Systems Considering Short-Term Operation Performance

Energies
Integrating photovoltaic power stations into large-capacity hydropower stations is an efficient and promising method for regulating large-scale photovoltaic power generation.
Wenlin Yuan, Zhangchi Sun
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source