Results 71 to 80 of about 34,748 (264)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Manipulation and optimisation techniques for Boolean logic

IET Computers & Digital Techniques, 2010
In this study, new techniques and algorithms are presented for the derivation and optimisation of mixed polarity Reed Muller (MPRM) and mixed polarity dual Reed Muller (MPDRM) functions. The first algorithm is used for bidirectional conversion between fixed polarity dual Reed Muller (FPDRM) and MPDRM and to derive any polarity from another polarity ...
Al Jassani, B.A., Urquhart, N., Almaini, A.E.A.   +2 more
openaire   +2 more sources

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach

FEBS Letters, EarlyView.
The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring, Jan de Jonge, Hans‐Peter Braun, Nils Rugen   +3 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Optimising Graphical Techniques Applied to Irreversible Tracers

Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies, 2018
Graphical analysis techniques are often applied to positron emission tomography (PET) images to estimate\ud physiological parameters. Patlak analysis is primarily used to obtain the rate constant (Ki) that indicates the\ud transfer of a tracer from plasma to the irreversible compartment and ultimately describes how the tracer\ud binds to the targeted ...
Yasser Alzamil, Yulia Hicks, Xin Yang 0033, Christopher Marshall   +3 more
openaire   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Advancements and Optimisation Strategies in Building Integrated Photovoltaic Thermal (BIPVT) Systems [PDF]

Iranian Journal of Electrical and Electronic Engineering
This paper provides an overview of the current innovations in Building Integrated Photovoltaic Thermal Systems. This paper briefly describes varying performance evaluation techniques, optimisation techniques, and the environmental impact and cost ...
Mon Prakash Upadhyay, Arjun Deo, Ajitanshu Vedratnam   +2 more
doaj  

Branch Optimisation Techniques for Hardware Compilation

, 2003
This paper explores using information about program branch probabilities to optimise reconfigurable designs. The basic premise is to promote utilization by dedicating more resources to branches which execute more frequently. A hardware compilation system has been developed for producing designs which are optimised for different branch probabilities. We
Henry Styles, Wayne Luk
openaire   +2 more sources

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source