Results 21 to 30 of about 517,318 (306)

Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity

BMC Biology, 2022
Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system. Features such as recognition of T-rich protospacer-adjacent motif (PAM) and generation of sticky breaks, as ...
Hongxin Huang, Guanjie Huang, Zhihong Tan, Yongfei Hu, Lin Shan, Jiajian Zhou, Xin Zhang, Shufeng Ma, Weiqi Lv, Tao Huang, Yuchen Liu, Dong Wang, Xiaoyang Zhao, Ying Lin, Zhili Rong   +14 more
doaj   +1 more source

Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathwayResearch in context

EBioMedicine, 2019
Background: We previously found that loss of lncRNA-AZIN2 splice variant (AZIN2-sv) increases cardiomyocyte (CM) proliferation and attenuates adverse ventricular remodelling post-myocardial infarction (MI).
Xinzhong Li, Yili Sun, Senlin Huang, Yanmei Chen, Xiaoqiang Chen, Mengsha Li, Xiaoyun Si, Xiang He, Hao Zheng, Lintao Zhong, Yang Yang, Wangjun Liao, Yulin Liao, Guojun Chen, Jianping Bin   +14 more
doaj   +1 more source

Frequency and functional translation of low muscle mass in overweight and obese patients with COPD

Respiratory Research, 2021
Background Cut offs for fat-free mass index (FFMI) and appendicular skeletal muscle mass index (ASMI) are available for diagnosing low muscle mass in patients with COPD. This study aimed to investigate: (1) the frequency of low muscle mass (FFMI and ASMI)
Felipe V. C. Machado, Martijn A. Spruit, Miriam T. J. Groenen, Sarah Houben-Wilke, Paula P. van Melick, Nidia A. Hernandes, Annemie M. W. J. Schols, Fabio Pitta, Emiel F. M. Wouters, Frits M. E. Franssen   +9 more
doaj   +1 more source

Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway

Gut Microbes, 2021
Microbial factors that mediate microbes-host interaction in ulcerative colitis (UC), a chronic disease seriously affecting human health, are not fully known. The emerging oncobacterium Fusobacterium nucleatum (Fn) secretes extracellular vesicles carrying
Le Liu, Liping Liang, Chenghai Yang, Youlian Zhou, Ye Chen   +4 more
doaj   +1 more source

Identification of Radiotherapy-Associated Genes in Lung Adenocarcinoma by an Integrated Bioinformatics Analysis Approach

Frontiers in Molecular Biosciences, 2021
Radiotherapy (RT) plays an important role in the prognosis of lung adenocarcinoma (LUAD) patients, but the radioresistance (RR) of LUAD is still a challenge that needs to be overcome.
Junhao Wang, Qizheng Han, Huizi Liu, Haihua Luo, Lei Li, Aihua Liu, Yong Jiang   +6 more
doaj   +1 more source

A CRM1 Inhibitor Alleviates Cardiac Hypertrophy and Increases the Nuclear Distribution of NT-PGC-1α in NRVMs

Frontiers in Pharmacology, 2019
Chromosomal maintenance 1 (CRM1) inhibitors display antihypertrophic effects and control protein trafficking between the nucleus and the cytoplasm. PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1alpha) is a type of transcriptional ...
Zuheng Liu, Zuheng Liu, Haiping Tian, Haiping Tian, Haiping Tian, Jinghai Hua, Jinghai Hua, Wanqiang Cai, Wanqiang Cai, Yujia Bai, Yujia Bai, Qiong Zhan, Qiong Zhan, Wenyan Lai, Wenyan Lai, Qingchun Zeng, Qingchun Zeng, Hao Ren, Hao Ren, Dingli Xu, Dingli Xu   +20 more
doaj   +1 more source

Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair

Nature Communications, 2022
G2/M arrest of epithelial cells leads to fibrosis with unclear mechanisms. This study identifies a protein-encoding circRNA, circBNC2, which inhibits epithelial cells G2/M arrest to prevent fibrotic maladaptive repair in damaged kidney and liver ...
Peng Wang, Zhitao Huang, Yili Peng, Hongwei Li, Tong Lin, Yingyu Zhao, Zheng Hu, Zhanmei Zhou, Weijie Zhou, Youhua Liu, Fan Fan Hou   +10 more
doaj   +1 more source

Clinical Characteristics and Prognostic Risk Factors for Pediatric B‐Cell Lymphoblastic Lymphoma: A Multicenter Retrospective Cohort Study for China Net Childhood Lymphoma

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background B‐cell lymphoblastic lymphoma (B‐LBL) represents a rare variety of non‐Hodgkin lymphoma, with limited research on its biology, progression, and management. Methods A retrospective analysis was performed on the clinical characteristics of 256 patients aged ≤18 years who received treatment under the China Net Childhood Lymphoma (CNCL)‐
Zhijuan Liu, Jian Wang, Ling Jin, Yanlong Duan, Fu Li, Yueping Jia, Leping Zhang, Xiaojun Yuan, Wei Liu, Ying Liu, Ansheng Liu, Mincui Zheng, Yunpeng Dai, Lian Jiang, Lirong Sun, Xiaowen Zhai, Rong Liu, Chunju Zhou, Zifen Gao, Yonghong Zhang, Xiaomei Yang   +20 more
wiley   +1 more source

The top tertile of hematocrit change during hospitalization is associated with lower risk of mortality in acute heart failure patients

BMC Cardiovascular Disorders, 2017
Background Hemoconcentration has been proposed as surrogate for changes in volume status among patients hospitalized with acute heart failure (AHF) and is associated with a favorable outcome.
Haobin Zhou, Tianyu Xu, Yuli Huang, Qiong Zhan, Xingfu Huang, Qingchun Zeng, Dingli Xu   +6 more
doaj   +1 more source

Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Communications Biology, 2021
Hongxin Huang and Yongfei Hu et al. develop Tag-seq, a streamlined sequencing method to examine off-target editing by CRISPR-Cas systems. The authors validate Tag-seq in multiple cell lines, demonstrating its broad utility and flexibility in identifying ...
Hongxin Huang, Yongfei Hu, Guanjie Huang, Shufeng Ma, Jianqi Feng, Dong Wang, Ying Lin, Jiajian Zhou, Zhili Rong   +8 more
doaj   +1 more source