Results 181 to 190 of about 587,545 (300)

Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli

open access: yesJournal of Bacteriology, 1984
D. Rawlings, I. Pretorius, D. Woods
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication

open access: yesJournal of Virology, 1983
P. Tegtmeyer   +5 more
semanticscholar   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

open access: yesFEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito   +3 more
wiley   +1 more source

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