Humanizing the yeast origin recognition complex [PDF]
Nature Communications, 2021 In most model yeast species the Origin Recognition Complex (ORC) binds defined and species-specific base sequences while in humans what determines the binding appears to be more complex.
Clare S. K. Lee +9 more
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The dynamic nature of the human origin recognition complex revealed through five cryoEM structures [PDF]
eLife, 2020 Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to origin DNA, recruiting CDC6, and assembling the MCM ...
Matt J Jaremko +4 more
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Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6 [PDF]
Nature Communications, 2020 The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its ...
Jan Marten Schmidt, Franziska Bleichert
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Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors. [PDF]
Eur J Hum Genet, 2019 Meier–Gorlin syndrome (MGS) is a rare, congenital primordial microcephalic dwarfism disorder. MGS is caused by genetic variants of components of the origin recognition complex (ORC) consisting of ORC1–6 and the pre-replication complex, which together ...
Maerz LD +5 more
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Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation. [PDF]
Nucleic Acids Res, 2016 Initiation of DNA replication depends upon recognition of genomic sites, termed origins, by AAA+ ATPases. In prokaryotes a single factor binds each origin, whereas in eukaryotes this role is played by a six-protein origin recognition complex (ORC).
Marques CA +7 more
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Developmental regulation of the Tetrahymena thermophila origin recognition complex. [PDF]
PLoS Genetics, 2015 The Tetrahymena thermophila DNA replication machinery faces unique demands due to the compartmentalization of two functionally distinct nuclei within a single cytoplasm, and complex developmental program.
Po-Hsuen Lee +2 more
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Structure of the active form of human origin recognition complex and its ATPase motor module [PDF]
eLife, 2017 Binding of the Origin Recognition Complex (ORC) to origins of replication marks the first step in the initiation of replication of the genome in all eukaryotic cells.
Ante Tocilj +7 more
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The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure [PDF]
eLife, 2021 The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an
Hsiang-Chen Chou +12 more
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Origin recognition complex subunit 1(ORC1) is a potential biomarker and therapeutic target in cancer [PDF]
BMC Medical Genomics, 2023 Background The origin recognition complex 1 (ORC1) is a large subunit of the origin recognition complex and acts as the master subunit of the precoding complex. Objective To explore potential function and clinical significance of ORC1 in cancers. Methods
Linling Wu, Hui Chen, Chao Yang
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Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance [PDF]
Cancer Medicine, 2023 Background DNA replication alteration is a hallmark of patients with lung adenocarcinoma (LUAD) and is frequently observed in LUAD progression. Origin recognition complex (ORC) 1, ORC2, ORC3, ORC4, ORC5, and ORC6 form a replication–initiator complex to ...
Min Tang +6 more
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