Humanizing the yeast origin recognition complex [PDF]
Nature Communications, 2021 In most model yeast species the Origin Recognition Complex (ORC) binds defined and species-specific base sequences while in humans what determines the binding appears to be more complex.
Clare S. K. Lee +9 more
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The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans [PDF]
Biology, 2023 Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward ...
Bik-Kwoon Tye, Yuanliang Zhai
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The origin recognition complex in human diseases [PDF]
Bioscience Reports, 2013 ORC (origin recognition complex) serves as the initiator for the assembly of the pre-RC (pre-replication complex) and the subsequent DNA replication.
Zhen Shen
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The dynamic nature of the human origin recognition complex revealed through five cryoEM structures [PDF]
eLife, 2020 Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to origin DNA, recruiting CDC6, and assembling the MCM ...
Matt J Jaremko +4 more
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Nonreplicative functions of the origin recognition complex [PDF]
Nucleus, 2018 Origin recognition complex (ORC), a heteromeric six-subunit complex, is the central component of the eukaryotic pre-replication complex. Recent data from yeast, frogs, flies and mammals present compelling evidence that ORC and its individual subunits have nonreplicative functions as well.
Alexander Brechalov +2 more
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Developmental regulation of the Tetrahymena thermophila origin recognition complex. [PDF]
PLoS Genetics, 2015 The Tetrahymena thermophila DNA replication machinery faces unique demands due to the compartmentalization of two functionally distinct nuclei within a single cytoplasm, and complex developmental program.
Po-Hsuen Lee +2 more
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Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast [PDF]
Cells, 2022 Precise DNA replication is pivotal for ensuring the accurate inheritance of genetic information. To avoid genetic instability, each DNA fragment needs to be amplified only once per cell cycle.
Karolina Stępień +4 more
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The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure [PDF]
eLife, 2021 The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an
Hsiang-Chen Chou +12 more
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Origin recognition complex subunit 1(ORC1) is a potential biomarker and therapeutic target in cancer [PDF]
BMC Medical Genomics, 2023 Background The origin recognition complex 1 (ORC1) is a large subunit of the origin recognition complex and acts as the master subunit of the precoding complex. Objective To explore potential function and clinical significance of ORC1 in cancers. Methods
Linling Wu, Hui Chen, Chao Yang
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Architecture of the Human Origin Recognition Complex [PDF]
Journal of Biological Chemistry, 2001 All the human homologs of the six subunits of Saccharomyces cerevisiae origin recognition complex have been reported so far. However, not much has been reported on the nature and the characteristics of the human origin recognition complex. In an attempt to purify recombinant human ORC from insect cells infected with baculoviruses expressing HsORC ...
S K, Dhar, L, Delmolino, A, Dutta
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