Results 211 to 220 of about 9,580 (245)

Anti-M1R/B6R antibody characterization and bispecific design for enhanced orthopoxvirus protection

EMBO Molecular Medicine
The global outbreak of the mpox in humans, caused by the mpox virus (MPXV), underscores the urgent need for safe and effective therapeutics. In this study, we characterized the dominant MPXV immunogens, M1R and B6R, by sequencing monoclonal antibodies ...
Runchu Zhao, Lili Wu, Yi Zhang, Jianrong Ma, Dezhi Liu, Yuxuan Zheng, Tianxiang Kong, Renyi Ma, Zhengrong Gao, Yan Chai, Yuanlang Liu, Yi Tian, Yunxia Xia, Yongzhi Hou, Jiahan Lu, Zhe Cong, Baoying Huang, Wenjie Tan, Jing Xue, G. Gao, Qihui Wang   +20 more
semanticscholar   +1 more source

Biosecurity Implications for the Synthesis of Horsepox, an Orthopoxvirus

Health Security, 2017
This article examines the biosecurity and biodefense implications resulting from the recent creation of horsepox virus, a noncirculating (extinct) species of orthopoxvirus. Here we examine the technical aspects of the horsepox virus synthesis and conclude that orthopox synthesis experiments currently remain technically challenging-and will continue to ...
Diane, DiEuliis, Kavita, Berger, Gigi, Gronvall   +2 more
openaire   +2 more sources

Genome Sequences of Akhmeta Virus, an Early Divergent Old World Orthopoxvirus

Viruses, 2018
Annotated whole genome sequences of three isolates of the Akhmeta virus (AKMV), a novel species of orthopoxvirus (OPXV), isolated from the Akhmeta and Vani regions of the country Georgia, are presented and discussed. The AKMV genome is similar in genomic
Jinxin Gao, Crystal M Gigante, Kimberly Wilkins   +2 more
exaly   +2 more sources

Development of a multiplex real-time PCR assay for the simultaneous detection of mpox virus and orthopoxvirus infections.

Journal of Virological Methods
Since May 2022, the multi-country outbreak of monkeypox (mpox) has raised a great concern worldwide. Early detection of mpox virus infection is recognized as an efficient way to prevent mpox transmission.
Zhangling Fan, Yu Xie, Baoying Huang, Fei Zhao, Yamei Hu, Yu Huang, S. Mei, Liang Wei, Liming Wang, Lingwa Wang, Zhao Gao, Bin Ai, J. Fang, Chen Liang, Fengwen Xu, Wenjie Tan, Fei Guo   +16 more
semanticscholar   +1 more source

Antibodies to orthopoxvirus in domestic cats in Norway

Veterinary Record, 1998
The prevalence of antibodies to orthopoxvirus in 217 sera collected from domestic cats in the western part of Norway was 10.1 per cent as measured by a competitive ELISA. In one of the seropositive cats antibodies were also detected by an immunofluorescence assay.
M, Tryland, T, Sandvik, L, Holtet, H, Nilsen, O, Olsvik, T, Traavik   +5 more
openaire   +2 more sources

Orthopoxvirus vaccines and vaccination

2007
Immunization procedures against Variola virus, from the historical perspective most often first credited to Edward Jenner in the late 18th century, helped finally to eradicate smallpox from the world. Since its eradication, the study of this disease and its pathology has been given little attention; however, with the emergence of Monkeypox virus into ...
Lauren M. Handley, J. Paige Mackey, R. Mark Buller, Clifford J. Bellone   +3 more
openaire   +1 more source

Orthopoxvirus pan-genomic DNA assay

Journal of Virological Methods, 2007
A genome-spanning assay is described that enables laboratory confirmation of infections with orthopoxviruses (OPVs), particularly Vaccinia, Monkeypox, and Variola viruses, which can cause vesiculo-pustular rash illnesses in humans. The assay uses a series of polymerase chain reaction (PCR) amplicons that overlap to span the approximately 200kilobase ...
Yu, Li, Susan L, Ropp, Hui, Zhao, Inger K, Damon, Joseph J, Esposito   +4 more
openaire   +2 more sources

Two types of deletions in orthopoxvirus genomes

Virus Genes, 1995
The genome nucleotide sequences of two strains of variola major virus and one strain of vaccinia virus were compared. One hundred and sixty-eight short (less than 100 bp in length) and eight long (more than 900 bp in length) deletions, four deletion/insertion regions, and four regions of multiple mutational differences between variola and vaccinia ...
S N, Shchelkunov, A V, Totmenin
openaire   +2 more sources

Regulation of Orthopoxvirus Gene Expression

1990
Poxviruses differ from the other major groups of DNA viruses with regard to their use of the cytoplasm, rather than the nucleus, as the site of transcription. Consistent with this strategy, poxviruses encode their own DNA-dependent RNA polymerase as well as many—if not all—of the additional enzymes and factors needed to synthesize functional capped ...
openaire   +2 more sources

Inactivation of orthopoxvirus for diagnostic PCR analysis

Journal of Virological Methods, 2007
Diagnoses of ongoing viral infections commonly rely on PCR methodology. Sample material that may contain hazardous virus should be efficiently inactivated in biological containment or bed-side before diagnostic PCR analysis. Surprisingly little documentation is available for inactivation of human viral pathogens by inactivation reagents that allow for ...
Lasse, Vinner, Anders, Fomsgaard
openaire   +2 more sources