Results 111 to 120 of about 60,322 (306)

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

Biotechnology Progress, EarlyView.
Abstract One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N‐1 stage) to seed the production culture (N stage) at ultra‐high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves cell culture performance by shortening the timeline and/or achieving higher final
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Osmosis, colligative properties, entropy, free energy and the chemical potential [PDF]

arXiv, 2014
A diffusive model of osmosis is presented that explains currently available experimental data. It makes predictions that distinguish it from the traditional convective flow model of osmosis, some of which have already been confirmed experimentally and others have yet to be tested.
arxiv  

An investigation into corneal enzymatic resistance following epithelium-off and epithelium-on corneal cross-linking protocols [PDF]

, 2016
The aim of this study was to investigate corneal enzymatic resistance following epithelium off and on riboflavin/UVA cross-linking (CXL). One hundred and fourteen porcine eyes were divided into four non-irradiated control groups and seven CXL groups. The
Aldahlawi, Nada Hussein, Hayes, Sally, Meek, Keith Michael Andrew, O'Brart, David P.S., O'Brart, Naomi   +4 more
core   +1 more source

Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies

Biotechnology Progress, EarlyView.
Abstract For bioprocesses producing live virus, such as enterovirus Coxsackievirus A21, viral titer (infectivity basis) decay rates can exceed 30% within a day. Consequently, harvest timing is paramount. To optimize titer at harvest, a continuous viral product titer model was generated to elucidate kinetics.
Katherine Forrester, Thomas R. Blanda, Marena Trauger, Rachel Thompson, Neil Templeton   +4 more
wiley   +1 more source

Acute severe thrombocytopenia following non-ionic low-osmolarity intravenous contrast medium injection [PDF]

, 2012
Intravenous contrast medium (ICM) rarely induces anaphylactic reactions, including urticaria, hypotension and respiratory failure. Even the most modern ICM may cause such adverse events.
Adam Domonkos Tarnoki, Aster, Bonnard, Chang, David Laszlo Tarnoki, Evelin Horvath, Ferenc Szalay, Hunt, Lacy, Pal Bata, Schulze, Shojania, Ural, Viktor Berczi, Wein, Wiemer   +15 more
core   +1 more source

A recurrent neural network for soft sensor development using CHO stable pools in fed‐batch process for SARS‐CoV‐2 spike protein production as a vaccine antigen

Biotechnology Progress, EarlyView.
Abstract Fed‐batch recombinant therapeutic protein (RTP) production processes utilizing Chinese Hamster Ovary (CHO) cells can take a long period of time (>10 days). Within this period, not all critical features may be measured routinely, and in fact, some are only measured once the process is terminated, complicating decision making.
Sebastian‐Juan Reyes, Robert Voyer, Yves Durocher, Olivier Henry, Phuong Lan Pham   +4 more
wiley   +1 more source

Glial Versus Neuronal Na+/K+‐ATPase in Activity‐Evoked K+ Clearance and Their Sensitivity to Elevated Extracellular K+

Glia, EarlyView.
The glial Na+ pump regulates [K+]o during neuronal activity, and the neuronal version restores [K+]o to baseline. Spreading depression‐related rise of K+ does not cause SD propagation via suppression of Na+/K+‐ATPase or glutamate transport. ABSTRACT Neuronal activity in the central nervous system is associated with a [K+]o transient that is swiftly ...
Brian Skriver Nielsen, Brian Roland Larsen, Afnan Bilal Ghazal, Adriana Katz, K. C. Brennan, Steven J. D. Karlish, Nanna MacAulay   +6 more
wiley   +1 more source

Physically crosslinked gelatin bio‐inks with enhanced printability, degradation and mechanical robustness for multi‐modal bioprinting

Interdisciplinary Medicine, EarlyView.
A sustainable, physically crosslinked bio‐ink (Gel‐X) composed of gelatin, gellan gum, and Laponite XLG was developed for multi‐modal 3D bioprinting. Gel‐X is prepared within an hour by simple mixing in deionized water, eliminating the need for chemical crosslinkers.
Wei Long Ng, Chuen Herh Ethan Yeow, Xi Huang, Samuel Lee, Wai Yee Yeong   +4 more
wiley   +1 more source

Biodegradation, mechanical and biocompatibility evaluation of compact and porous polylactic acid after long‐term dynamic fluid immersion

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Polylactic acid (PLA) is the most widely used thermoplastic in applications in the benefit of humans, such as biomedicine and food packaging. However, its behaviour when operating in relevant environments has not been fully investigated.
DV Portan, A Angelopoulou, A Koliadima, LC Kontaxis, G Michanetzis, D Kouzoudis, E Michalopoulos, GC Papanicolaou   +7 more
wiley   +1 more source

Connecting the dots between mechanosensitive channel abundance, osmotic shock, and survival at single-cell resolution [PDF]

arXiv, 2018
Rapid changes in extracellular osmolarity are one of many insults microbial cells face on a daily basis. To protect against such shocks, Escherichia coli and other microbes express several types of transmembrane channels which open and close in response to changes in membrane tension. In E.
arxiv