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Osteoblast cell membrane hybrid bilayers for studying cell–cell interactions
IEE Proceedings - Nanobiotechnology, 2004Osteoblast-like cells were grown on a surface that presents cell membrane components to the cells in culture. The culture surface was a bimolecular layer formed by the interaction of osteoblast plasma membrane vesicles with an alkanethiol monolayer. The potential of these osteoblast-membrane hybrid bilayers for promoting osteoblast adhesion, growth and
J T, Elliott +5 more
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Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells
Biochemical and Biophysical Research Communications, 2004Regulation of the bone sialoprotein (BSP) gene is important in the differentiation of osteoblasts, in bone matrix mineralization, and in tumor metastasis. We investigated BSP gene transcription by performing functional analysis of the 9256bp of the 5' flanking region of the murine BSP gene containing its promoter. We found that the forced expression of
Qisheng, Tu +3 more
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The Intrinsic Incompressibility of Osteoblast-like Cells
Tissue Engineering, 1996This paper presents a new methodology, apparatus design, and the experimental results of ongoing research into the measurement of the mechanical properties of musculoskeletal tissue at the cellular level. A microchamber was constructed that provides a controlled hydrostatic pressure environment for these cells where optical sectioning, via ...
R P, Wilkes, K A, Athanasiou
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Whole-Cell Membrane Currents from Human Osteoblast-like Cells
Calcified Tissue International, 1998Bone cells share common responses to external stimuli with most other cells. Among these are changes in membrane ion channel activity, although at present, relatively little is known about their nature or significance in human bone cells. Using the whole-cell configuration of the patch-clamp technique, we have revealed two types of membrane current in ...
C E, Yellowley +3 more
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OSTEOBLASTIC CELLS ARE INVOLVED IN OSTEOCLAST FORMATION
Endocrinology, 1988We developed a co-culture system with mouse spleen cells and osteoblastic cells to examine the role of osteoblasts in osteoclast formation. When mouse spleen cells and osteoblastic cells isolated from fetal mouse calvariae were co-cultured in the presence of 10 nM 1 alpha, 25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], numerous tartrate-resistant acid ...
N, Takahashi +7 more
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Steroid receptors in human osteoblast-like cells
European Journal of Cancer and Clinical Oncology, 1990The presence and functions of steroid receptors were evaluated in three human osteosarcoma cell lines (OS1 = SA OS; OS2 = HOS TE 85, and OS3 = MNNG HOS TE 85). The human breast cancer cell line MCF-7 was used as internal control for oestrogen receptors (E2R). High and low affinity sites were characterised.
M C, Etienne +7 more
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Primary cortical brain cells influence osteoblast activity
Biochemical and Biophysical Research Communications, 2009The presence of neuropeptides and neuroreceptors in the bone have been reported in several studies. Bone turn-over seems to be controlled by the nervous system. The actual pathway or the control mechanism is still under investigation. In this study we investigate the changes in osteoblast cells if they are in co-culture with primary cortical brain ...
Lucas, Anissian +2 more
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Gene expression in osteoblastic cells.
Critical reviews in eukaryotic gene expression, 1992Osteoblasts, the bone-forming cells, synthesize the macromolecules of the bone matrix including: type I collagen; osteocalcin; osteonectin; osteopontin; proteoglycan I and II; bone sialoprotein; matrix gla-protein; bone glycoprotein 75; several other proteins, which have not been extensively characterized; growth factors, including transforming growth ...
G A, Rodan, M, Noda
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