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Isolation and Culture of Osteoblasts
This chapter describes the isolation, culture, and staining of primary osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis.
Lucie E, Bourne, Isabel R, Orriss
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Isolation and Generation of Osteoblasts
, 2019 This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis.
Inês P, Perpétuo +2 more
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Advances in the osteoblast lineage
Biochemistry and Cell Biology, 1998Osteoblasts are the skeletal cells responsible for synthesis, deposition and mineralization of the extracellular matrix of bone. By mechanisms that are only beginning to be understood, stem and primitive osteoprogenitors and related mesenchymal precursors arise in the embryo and at least some appear to persist in the adult organism, where they ...
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Osteoblast and chondroblast differentiation
Bone, 1995Recognition of discrete commitment and differentiation stages requires characterization of changes in proliferative capacity together with the temporal acquisition or loss of expression of molecular and morphological traits. Both cell lines and primary cultures have been useful for analysis of transitional steps in the chondroblast (CB) and osteoblast (
J E, Aubin +3 more
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Osteoblast function in myeloma
Bone, 2011Multiple myeloma (MM) is the most frequent cancer to involve the skeleton and results in purely osteolytic lesions that rarely heal. MM bone disease is responsible for some of the most devastating complications of MM. The marrow microenvironment plays a key role in MM bone disease as well as in the initiation, expansion and chemoresistance of MM cells.
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Properties and Origin of Osteoblasts
Clinical Orthopaedics and Related Research, 1990Osteoblastic and chondroblastic (i.e., osteogenic) cells belong to the stromal cell system, which is associated with bone marrow, and bone and is separate from the hematopoietic stem-cell system. Stromal stem cells are capable of producing reticular, fibroblastic, osteogenic, and adipose stromal lines. Marrow-derived osteogenic cells are a component of
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Notch Signaling in Osteoblasts
Science Signaling, 2008Bone remodeling is the result of the coordinated activity of osteoblasts, which form new matrix, and osteoclasts, which resorb bone. Notch proteins are single-pass transmembrane receptors that determine cell fate. Recent gain-of-function and loss-of-function experiments reveal a suppressive effect of Notch in osteoblast and osteoclast differentiation ...
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The diverse origin of bone-forming osteoblasts
Journal of Bone and Mineral Research, 2021Toshihide Mizoguchi, Noriaki Ono
exaly