A Laboratory Exercise Simulating Antibody and Antigen Reactions of the Ouchterlony Double Immunodiffusion Assay Using Inorganic Salts [PDF]
Journal of Microbiology & Biology Education, 2021 The Ouchterlony double immunodiffusion assay is a serological technique used in the detection of antibodies and antigens for diagnostic purposes and also used in immunology laboratory courses as a common teaching assay where students observe the ...
Mustafa G. Mujtaba +3 more
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Detection of anti- SpA antibodies in egg white tested by double immunodiffusion (Ouchterlony) technique. v1 [PDF]
, 2020 Keyhole limpet hemocyanin (KLH) is a cooper-containing protein comprising of subunits with MW of 400 kDa. This protein is found in the hemolymph of the sea mollusk Megathura crenulata. It has the ability to enhance the host’s immune response by interacting with monocytes, T cells and macrophages.
Angel A Justiz-Vaillant
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Production of polyclonal antibody against the recombinant PirBvp protein of Vibrio parahaemolyticus [PDF]
Journal of Genetic Engineering and Biotechnology, 2021 Background Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirAvp and PirBvp encoded in pVA1 plasmid.
Ngoc-Diem Duong +5 more
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Binding of avian immunoglobulins to peroxidase-labeled anti-chicken IgY conjugate by double immunodiffusion (Ouchterlony) technique. v1 [PDF]
, 2020 In this experiment anti-chicken IgY developed in rabbit cross-reacts with a large panel of avian species including ostrich, duck, pigeon, and quail.
Angel A Justiz-Vaillant
openaire +2 more sources
Binding properties of Human IgG to immunoglobulin-binding proteins tested by double immunodiffusion (Ouchterlony) technique. v1 [PDF]
, 2020 1. IBP-HRP conjugates can be prepared in the laboratory by the periodate method. 2. The Human IgG used can be commercially available of prepared in the laboratory from human sera by affinity chromatography (using an SpG-agarose column). 3. SpG-agarose column are widely commercially prepared. 4.
Angel A Justiz-Vaillant
openaire +2 more sources
Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488 [PDF]
Biomolecules, 2020 The Pantoea agglomerans 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation.
Tetiana V. Bulyhina +6 more
doaj +2 more sources
Characterization of Tunga penetrans antigens in selected epidemic areas in Murang'a county in Kenya. [PDF]
PLoS Neglected Tropical Diseases, 2015 Tunga penetrans are fleas that cause tungiasis, a condition characterized by high transmission rate due to poor housing conditions, social neglect and inadequate health care in economically disadvantaged communities in developing countries.
Jamleck N Mwangi +3 more
doaj +2 more sources
Binding properties of immunoglobulin-binding proteinby double immunodiffusion (Ouchterlony) technique. v1 [PDF]
, 2020 Angel A Justiz-Vaillant
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Extraction and quantification of antivenomous antibodies in chicken eggs against scorpion venom
Ciência Rural, 2023 : Avian-derived IgY is thought to be the best therapy for scorpion bites concerning low-level side effects. The present study analyzed a hypothesis about the neutralization of scorpion venom Androcotonus australis through antibodies produced in the egg ...
Mehvish Andleeb, Aleem Ahmed Khan
doaj +1 more source
SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOPOLYSACCHARIDE FROM Escherichia coli L-19 [PDF]
Biotechnologia Acta, 2015 The lipopolysaccharide from Escherichia coli L-19 was isolated, chemically identified and its nontoxicity but pyrogenicity were shown. The investigations of lipopolysaccharide influence on T- and B-lymphocytes indicated that it might be used as a mitogen
L. D. Varbanets +3 more
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