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Outer Membrane Proteins of Pathogenic Spirochetes. [PDF]

open access: yesFEMS Microbiology Reviews, 2004
Pathogenic spirochetes are the causative agents of several important diseases including syphilis, Lyme disease, leptospirosis, swine dysentery, periodontal disease and some forms of relapsing fever. Spirochetal bacteria possess two membranes and the proteins present in the outer membrane are at the site of interaction with host tissue and the immune ...
Ben Adler, David A Haake, Paul A Cullen
exaly   +3 more sources
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Folding of outer membrane proteins

Archives of Biochemistry and Biophysics, 2013
Outer membrane proteins (OMPs) represent a large group of β-barrel proteins found both in the membranes of both bacteria and eukaryotes. Their general ease of expression and refolding and straightforward methods to monitor their degree of folding conspire to make OMPs excellent model systems to investigate how the membrane environment and other ...
Otzen, Daniel, Andersen, Kell K
openaire   +3 more sources

Outer Membrane Protein Purification

2003
The major outer membrane proteins (OMPs) from Neisseria meningitidis, which are expressed at high levels, are subdivided in five classes based on molecular weight (1,2) (see Table 1). Table 1 Major Meningococcal Outer-Membrane Proteins Outer-membrane proteins Name Molecular maass Function/characteristics Class 1 PorA 44-47 kDa Porin Class 2/3 PorB 37 ...
Arigita, C   +3 more
openaire   +4 more sources

Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine

Vaccine, 2020
The Chlamydial outer membrane complex (COMC) from the elementary body (EB) is a protein rich insoluble outer membrane shell from which cytosolic proteins have been extracted with detergent. In this study we conducted mass spectrometry experiments to detect proteins in the COMC prepared from C. muridarum EB.
Hong, Yu   +7 more
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Outer Membrane Proteins

1998
The membrane of Gram-negative bacteria consists of three layers: (i) the cytoplasmic or inner membrane, (ii) the peptidoglycan layer and (iii) the outer membrane (Figure 1). The cytoplasmic membrane consists of a phospholipid bilayer in which proteins are present which can play a role in solute transport, generation of energy and synthesis of cell ...
Robert E. W. Hancock   +1 more
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Biogenesis of Outer Membrane Proteins

2003
The cell envelope of Gram-negative bacteria consists of two membranes, the inner membrane, which is a phospholipid bilayer, and the outer membrane, which is an asymmetrical bilayer with phospholipids and lipopolysaccharides (LPS) in the inner and outer monolayers, respectively. The membranes are separated by the peptidoglycan-containing periplasm. Both
Tommassen, J.P.M., Voulhoux, J.R.
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Protein sorting between mitochondrial outer and inner membranes. Insertion of an outer membrane protein into the inner membrane

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1992
The amino terminal 29 amino acids of the outer mitochondrial membrane protein of yeast, OMM70 (MAS70), consisting of the targeting and membrane anchor domains, has been fused to a reporter protein, dihydrofolate reductase. The hybrid protein, designated pOMD29, was efficiently imported into the outer membrane of rat heart mitochondria by a process ...
J M, Li, G C, Shore
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Assembly of Bacterial Outer Membrane Proteins

2012
Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane proteins (OMPs). Here, we describe three biochemical methods that can be used in such studies to evaluate the proper assembly of OMPs into the ...
Grijpstra, J.   +2 more
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Outer membrane proteins of Pasteurella multocida

Veterinary Microbiology, 2010
Pasteurella multocida is a ubiquitous pathogen which causes a range of diseases in diverse animal species. Components of the bacterial outer membrane, such as trans membrane proteins and lipoproteins, play key roles in the interaction of the pathogen with the host environment and in the host immune response to infection. In this review, we evaluate the
Tamás, Hatfaludi   +3 more
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Outer Membrane Proteins

2010
The outer membrane is considered to be a barrier in Gram-negative bacteria. However, bacteria are not autonomous and have to communicate in many ways with their surroundings. This interaction ranges from their association to surfaces, uptake of solutes, and sensing of environmental conditions to export of solutes into attacked cells.
Oliver Mirus   +2 more
openaire   +1 more source

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