Results 321 to 330 of about 590,456 (352)
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1998
The membrane of Gram-negative bacteria consists of three layers: (i) the cytoplasmic or inner membrane, (ii) the peptidoglycan layer and (iii) the outer membrane (Figure 1). The cytoplasmic membrane consists of a phospholipid bilayer in which proteins are present which can play a role in solute transport, generation of energy and synthesis of cell ...
Robert E. W. Hancock +1 more
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The membrane of Gram-negative bacteria consists of three layers: (i) the cytoplasmic or inner membrane, (ii) the peptidoglycan layer and (iii) the outer membrane (Figure 1). The cytoplasmic membrane consists of a phospholipid bilayer in which proteins are present which can play a role in solute transport, generation of energy and synthesis of cell ...
Robert E. W. Hancock +1 more
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Outer Membrane Protein Purification
2003The major outer membrane proteins (OMPs) from Neisseria meningitidis, which are expressed at high levels, are subdivided in five classes based on molecular weight (1,2) (see Table 1). Table 1 Major Meningococcal Outer-Membrane Proteins Outer-membrane proteins Name Molecular maass Function/characteristics Class 1 PorA 44-47 kDa Porin Class 2/3 PorB 37 ...
Arigita, C +3 more
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Biochimica et Biophysica Acta (BBA) - Biomembranes, 1992
The amino terminal 29 amino acids of the outer mitochondrial membrane protein of yeast, OMM70 (MAS70), consisting of the targeting and membrane anchor domains, has been fused to a reporter protein, dihydrofolate reductase. The hybrid protein, designated pOMD29, was efficiently imported into the outer membrane of rat heart mitochondria by a process ...
J M, Li, G C, Shore
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The amino terminal 29 amino acids of the outer mitochondrial membrane protein of yeast, OMM70 (MAS70), consisting of the targeting and membrane anchor domains, has been fused to a reporter protein, dihydrofolate reductase. The hybrid protein, designated pOMD29, was efficiently imported into the outer membrane of rat heart mitochondria by a process ...
J M, Li, G C, Shore
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Biogenesis of Outer Membrane Proteins
2003The cell envelope of Gram-negative bacteria consists of two membranes, the inner membrane, which is a phospholipid bilayer, and the outer membrane, which is an asymmetrical bilayer with phospholipids and lipopolysaccharides (LPS) in the inner and outer monolayers, respectively. The membranes are separated by the peptidoglycan-containing periplasm. Both
Tommassen, J.P.M., Voulhoux, J.R.
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Outer membrane proteins of Pasteurella multocida
Veterinary Microbiology, 2010Pasteurella multocida is a ubiquitous pathogen which causes a range of diseases in diverse animal species. Components of the bacterial outer membrane, such as trans membrane proteins and lipoproteins, play key roles in the interaction of the pathogen with the host environment and in the host immune response to infection. In this review, we evaluate the
Tamás, Hatfaludi +3 more
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Assembly of Bacterial Outer Membrane Proteins
2012Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane proteins (OMPs). Here, we describe three biochemical methods that can be used in such studies to evaluate the proper assembly of OMPs into the ...
Grijpstra, J. +2 more
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2010
The outer membrane is considered to be a barrier in Gram-negative bacteria. However, bacteria are not autonomous and have to communicate in many ways with their surroundings. This interaction ranges from their association to surfaces, uptake of solutes, and sensing of environmental conditions to export of solutes into attacked cells.
Oliver Mirus +2 more
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The outer membrane is considered to be a barrier in Gram-negative bacteria. However, bacteria are not autonomous and have to communicate in many ways with their surroundings. This interaction ranges from their association to surfaces, uptake of solutes, and sensing of environmental conditions to export of solutes into attacked cells.
Oliver Mirus +2 more
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Outer-Membrane Proteins of Haemophilus paragallinarum
Avian Diseases, 1990The outer-membrane protein (OMP) profiles of four isolates of Haemophilus paragallinarum (0083, 0222, Modesto, and HP31) were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. OMPs were isolated by sonic disruption followed by differential centrifugation and selective solubilization in Triton X-100.
Blackall, P. J. +2 more
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Electroeluted Outer Membrane Proteins as Immunogens
Immunological Investigations, 1994Electroeluted outer membrane proteins [(EOmp), (35-37 KDa, porins)] were highly immunogenic in New Zealand White rabbits. An ELISA peak titer of 51,200 to EOmp as compared to 6,400 for non-eluted outer membrane protein (Omp) was demonstrated. EOmp enhanced the antigenicity of Omp possibly due to epitopes which were masked in the non-eluted antigen. Non-
M, Kawahara +3 more
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Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles
The Journal of Microbiology, 2012Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of ...
Dong Chan, Moon +7 more
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