Results 51 to 60 of about 590,456 (352)

Discrimination of outer membrane proteins with improved performance

BMC Bioinformatics, 2008
Background Outer membrane proteins (OMPs) perform diverse functional roles in Gram-negative bacteria. Identification of outer membrane proteins is an important task.
Hu Jing, Yan Changhui, Wang Yingfeng
doaj   +1 more source

Skp is a multivalent chaperone of outer-membrane proteins [PDF]

Nature Structural & Molecular Biology, 2016
The trimeric chaperone Skp sequesters outer-membrane proteins (OMPs) within a hydrophobic cage, thereby preventing their aggregation during transport across the periplasm in Gram-negative bacteria. Here, we studied the interaction between Escherichia coli Skp and five OMPs of varying size.
Schiffrin, B, Calabrese, AN, Devine, PWA, Harris, SA, Ashcroft, AE, Brockwell, DJ, Radford, SE   +6 more
openaire   +3 more sources

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Characterization of Inner and Outer Membrane Proteins from Francisella tularensis Strains LVS and Schu S4 and Identification of Potential Subunit Vaccine Candidates

mBio, 2017
Francisella tularensis is the causative agent of tularemia and a potential bioterrorism agent. In the present study, we isolated, identified, and quantified the proteins present in the membranes of the virulent type A strain, Schu S4, and the attenuated ...
Deborah M. B. Post, Bram Slütter, Birgit Schilling, Aroon T. Chande, Jed A. Rasmussen, Bradley D. Jones, Alexandria K. D’Souza, Lorri M. Reinders, John T. Harty, Bradford W. Gibson, Michael A. Apicella   +10 more
doaj   +1 more source

Import pathways of precursor proteins into mitochondria [PDF]

, 1988
The precursor of porin, a mitochondrial outer membrane protein, competes for the import of precursors destined for the three other mitochondrial compartments, including the Fe/S protein of the bc1- complex (intermembrane space), the ADP/ATP carrier ...
Neupert, Walter, Pfaller, Rupert, Pfanner, Nikolaus, Rassow, Joachim, Steger, Heinrich F.   +4 more
core  

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

The expression of outer membrane proteins for crystallization

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2003
The production of sufficient amounts of chemically and conformationally homogenous protein is a major requirement for successful crystallization and structure determination. With membrane proteins, this constitutes a particular problem because the membrane volume is limited and the organisms are usually very sensitive to changes in membrane properties ...
Bannwarth, Michael, Schulz, Georg E.
openaire   +3 more sources

Incorporation of Heterologous Outer Membrane and Periplasmic Proteins into Escherichia coli Outer Membrane Vesicles [PDF]

Journal of Biological Chemistry, 2004
Gram-negative bacteria shed outer membrane vesicles composed of outer membrane and periplasmic components. Since vesicles from pathogenic bacteria contain virulence factors and have been shown to interact with eukaryotic cells, it has been proposed that vesicles behave as delivery vehicles.
Nicole C, Kesty, Meta J, Kuehn
openaire   +2 more sources

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Anti-outer Membrane Vesicle Antibodies Increase Antibiotic Sensitivity of Pan-Drug-Resistant Acinetobacter baumannii

Frontiers in Microbiology, 2019
Acinetobacter baumannii often causes serious nosocomial infections. Because of its serious drug resistance problems, complex drug resistance mechanism, and rapid adaptation to antibiotics, it often shows pan-drug resistance and high fatality rates, which
Weiwei Huang, Qishu Zhang, Weiran Li, Yongjun Chen, Congyan Shu, Qingrong Li, Jingxian Zhou, Chao Ye, Hongmei Bai, Wenjia Sun, Xu Yang, Yanbing Ma   +11 more
doaj   +1 more source