Results 151 to 160 of about 51,375 (281)

Rapid Fabrication of Sub‐100 µm Microstructures In Situ via Femtosecond Laser for Correlative Imaging of Rare Cells

Small, EarlyView.
In this work, a femtosecond (fs) laser processing strategy is demonstrated for rapid and targeted fabrication of 3D sub‐100‐µm microstructures on resin‐embedded cells. The fabricated array of sub‐100‐µm pillars (each loaded with flow‐sorted T‐cell subsets) is arranged in a half‐grid geometry to enable efficient use of the scarce biological sample and ...
Aristide Djoulde, Yaping Chen, Vahid R. Adineh, Yang Liu, Boyin Liu, Di Yu, Jing Fu   +6 more
wiley   +1 more source

CryoBN‐PAGE: A Rapid and Cost‐Effective Method for the Analysis of Yeast Respiratory Complexes and Their Dynamic Organization

Yeast, EarlyView.
We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez, Mazzen H. Eldeeb, Elliana G. Mohrhardt, Flavia Fontanesi   +3 more
wiley   +1 more source

OxPhos Inhibitor VLX600 [PDF]

, 2020
openaire   +1 more source

Cenozoic Tectonics Ignite Mitochondrial Codon Innovations Propelling Canid Body Size Evolution and Transcontinental Radiations

Integrative Zoology, EarlyView.
We decode mitochondrial genomes across all extant canids, revealing lineage‐specific codon optimization driven by altitude, predation, and body size. A tripartite framework integrates geological events, metabolic constraints, and adaptive radiation to explain carnivore evolution.
Xiaoyang Wu, Xibao Wang, Yongquan Shang, Yao Chen, Haotian Cai, Tian Xia, Qinguo Wei, Weilai Sha, Honghai Zhang   +8 more
wiley   +1 more source

Serotonin Degradation and Lipid Metabolism Regulate Human Tc2 Cell Effector Functions

Allergy, EarlyView.
Human Tc2 cells exhibit a metabolically active phenotype characterized by enhanced glycolysis, mitochondrial respiration, and fatty acid uptake dependent on PPARγ. Alarmins strongly amplify IL‐5 and IL‐13 production in Tc2 cells, supporting a shared immunometabolic program across type 2 lymphocytes.
Sabrina de Souza Ferreira, Lisa Holla, Sophia Björkander, Marie Kaarup Bek, Lorenz Wirth, Patrick A. Shearer, Jesper Säfholm, Erik Sachs, Mamdoh Al‐Ameri, Kasra Vali, Sandra Ekström, Erik Melén, Jenny Mjösberg, Christopher Andrew Tibbitt   +13 more
wiley   +1 more source

Dendritic Cell α‐Ketoglutarate Regulates Tfh Polarization in Allergy

Allergy, EarlyView.
Allergen induces a dendritic cell metabolism shift toward glutaminolysis with α‐ketoglutarate as a regulator of Tfh subset polarization. Glutaminolysis is required for DC allergen uptake, co‐stimulatory molecule expression, and CLR expression. Modulation of glutaminolysis, JmjD3 histone demethylation, or HIF‐1α degradation alters Tfh polarization and ...
Anuj Tharakan, Xufeng Qu, Carmen Camarena, Araba Abaidoo‐Myles, Parth Naik, Jeremy Allegood, Ankit Kumar, Mihir Pokhriyal, Joseph C. Lownik, Matthew P. Zellner, Grayson W. Way, Jinze Liu, Lauren Ashley Cowart, Stephanie C. Eisenbarth, Joseph W. Landry, Daniel H. Conrad, Rebecca K. Martin   +16 more
wiley   +1 more source

Epigenetic training licenses naïve CD8⁺ T cell metabolic fitness and function. [PDF]

Sci Adv
Lv M, Ding J, Long H, Chai Q, Xu L, Jin C, Liang W, Zhang L, Zhang B, Lv Z, Zhao J, Li G, Zhu M.   +12 more
europepmc   +1 more source

Deficiency of Mitochondrial Fatty Acid Enzyme, CPT1A, Underlies Airway Epithelial Barrier Dysfunction in Severe Asthma

Allergy, EarlyView.
Mitochondrial fatty acid enzyme, CPT1A, is deficient in the airway epithelium of severe asthma patients. Restoration of CPT1A expression improved epithelial barrier integrity via increasing mitochondrial respiration and ATP production in airway epithelial cells. Restoration of deficient epithelial CPT1A may represent a new treatment approach for severe
Muyun Wang, Haiyang Hu, Kun Wang, Di Wu, Ximing Liao, Jing Gao, Tian Li, Wujian Xu, Shaoyong Gao, Qiang Li, Pankaj Kumar Bhavsar, Wei Gao, Kian Fan Chung   +12 more
wiley   +1 more source

The Role of Mitochondrial Dysfunction in Hepatocellular Carcinoma: From Pathogenesis and Drug Resistance to Targeted Therapeutic Strategies. [PDF]

J Hepatocell Carcinoma
Zhao S, Liu Q, Li J, Zhang S, Wan S, Yang Y, Jin F, Tuo B, Jin H.   +8 more
europepmc   +1 more source

Lactate‐Responsive Glycogen Dynamics Are Associated With Bull Sperm Motility and Fertilization Under Glucose‐Limited Conditions

Andrology, EarlyView.
ABSTRACT Background Mammalian sperm need to undergo hyperactivation, capacitation, and acrosome reaction to achieve fertilization competence. However, the female reproductive tract presents contrasting metabolic environments. The uterus is glucose‐rich, and the oviduct is lactate‐rich and glucose‐limited.
Md Faizul Hossain Miraz, Takahiro Yamanaka, Takashi Umehara, Shahrina Akter, Wambugu Enoc Njoroge, Masayuki Shimada   +5 more
wiley   +1 more source