Results 41 to 50 of about 24,345,005 (317)

Pyramidalization of the Glycosidic Nitrogen Provides the Way for Efficient Cleavage of the N‑Glycosidic Bond of 8‑OxoG with the hOGG1 DNA Repair Protein

, 2012
A mechanistic pathway for cleavage of the N-glycosidic bond of 8-oxo-2′-deoxyguanosine (oxoG) catalyzed with the human 8-oxoguanine glycosylase 1 DNA repair protein (hOGG1) is proposed in this theoretical study. The reaction scheme suggests direct proton
Sychrovský, V., Sub Cellular Protein Chemistry, Sebera, J., Tanaka, Y., Trantirek, L., Cellular Protein Chemistry   +5 more
core   +1 more source

Naturally acquired immune responses to P. vivax merozoite surface protein 3α and merozoite surface protein 9 are associated with reduced risk of P. vivax malaria in young Papua New Guinean children [PDF]

, 2013
Plasmodium vivax is the most geographically widespread human malaria parasite. Cohort studies in Papua New Guinea have identified a rapid onset of immunity against vivax-malaria in children living in highly endemic areas. Although numerous P.
Kiniboro, B, Stanisic, Danielle, Koepfli, Cristian, Koepfli, C, Cristian Koepfli, Felger, Ingrid, Balwan Singh, Meyer, Esmeralda V. S., Esmeralda V S Meyer, Kiniboro, Benson, Ingrid Felger, Jiang, J, Mary R Galinski, Singh, B, Javati, Sarah, Danielle I Stanisic, Stanisic, DI, Javati, S, Felger, I, Enmoore Lin, Mueller, Ivo, Siba, P, Ivo Mueller, Galinski, MR, Lin, Enmoore, Peter Siba, Lin, E, Siba, Peter, Mueller, I, Stanisic, Danielle I., Galinski, Mary R., Sarah Javati, Jianlin Jiang, Jiang, Jianlin, Meyer, EVS, Singh, Balwan, Benson Kiniboro   +36 more
core   +1 more source

Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp.

, 2012
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D., Stefan G D Rüdiger, Maurice, Madelon M., David P Minde, Sub Cellular Protein Chemistry, Stefan G. D. Rüdiger, Madelon M Maurice, Minde, David P., Cellular Protein Chemistry, David P. Minde, Madelon M. Maurice   +10 more
core   +1 more source

Insights into the Membranolytic Activity of Antimalarial Drug-Cell Penetrating Peptide Conjugates

Membranes, 2020
Conjugation of TP10, a cell-penetrating peptide with intrinsic antimalarial activity, to the well-known antimalarial drugs chloroquine and primaquine has been previously shown to enhance the peptide’s action against, respectively, blood- and liver-stage ...
Luísa Aguiar, Marina Pinheiro, Ana Rute Neves, Nuno Vale, Sira Defaus, David Andreu, Salette Reis, Paula Gomes   +7 more
doaj   +1 more source

Claudin‐6 Protein Expression in Atypical Teratoid/Rhabdoid Tumors Is Strongly Enriched in the Molecular Subgroup AT/RT‐TYR

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Claudin‐6 has emerged as a promising immunotherapeutic target, yet protein‐level data in atypical teratoid/rhabdoid tumors (AT/RTs) have been inconsistent. We analyzed 36 well‐characterized AT/RT samples and found membranous claudin‐6 protein expression in 58% of cases, with striking enrichment in the molecular subgroup AT/RT‐TYR (100%) and ...
Victoria E. Fincke, Christian Thomas, Michael C. Frühwald, Pascal D. Johann, Martin Hasselblatt   +4 more
wiley   +1 more source

Emapalumab for Immune Effector Cell‐Associated Hemophagocytic Lymphohistiocytosis‐Like Syndrome Following CD19‐Directed CAR‐T in Two Patients With B‐ALL: Clinical and Biomarker Correlates

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Immune effector cell‐associated hemophagocytic lymphohistiocytosis‐like syndrome (IEC‐HS) is a life‐threatening hyperinflammatory toxicity distinct from cytokine release syndrome (CRS) and neurotoxicity following chimeric antigen receptor T‐cell (CAR‐T) therapy. In a single‐institution retrospective cohort of pediatric and young adult patients
Thomas J. Galletta, Jeremy D. Rubinstein, Christopher E. Dandoy, Ruby Khoury, Angela Faulhaber, Christine L. Phillips, Christa Krupski   +6 more
wiley   +1 more source

Analysis of Disulfide Bond Formation [PDF]

, 2017
In this unit, protocols are provided for detection of disulfide bond formation in cultures of intact cells and in an in vitro translation system containing isolated microsomes or semi-permeabilized cells.
Sub Cellular Protein Chemistry, Ineke Braakman, Hebert, Daniel N., Lydia Lamriben, Guus van Zadelhoff, van Zadelhoff, Guus, Braakman, Ineke, Daniel N. Hebert, Lamriben, Lydia, Cellular Protein Chemistry   +9 more
core   +1 more source

Isoelectric point-amyloid formation of α-synuclein extends the generality of the solubility and supersaturation-limited mechanism

Current Research in Structural Biology, 2020
Proteins in either a native or denatured conformation often aggregate at an isoelectric point (pI), a phenomenon known as pI precipitation. However, only a few studies have addressed the role of pI precipitation in amyloid formation, the crystal-like ...
Koki Furukawa, Cesar Aguirre, Masatomo So, Kenji Sasahara, Yohei Miyanoiri, Kazumasa Sakurai, Keiichi Yamaguchi, Kensuke Ikenaka, Hideki Mochizuki, Jozsef Kardos, Yasushi Kawata, Yuji Goto   +11 more
doaj   +1 more source

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

Microbial Biotechnology, 2021
Summary We developed a cost sensitive isotope labelling procedure using a fed‐batch fermentation method and tested its efficiency producing the 15N‐, 13C‐ and 15N/13C‐labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS).
István Vida, Zsolt Fazekas, Gergő Gyulai, Dóra Nagy‐Fazekas, Gyula Pálfy, Pál Stráner, Éva Kiss, András Perczel   +7 more
doaj   +1 more source

The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli, Seth Rotz, Masatoshi Takagi, Richa Sharma   +3 more
wiley   +1 more source