Results 151 to 160 of about 20,634,842 (205)

Production of the human D2S receptor in the methylotrophic yeast P. pastoris.

Receptors and Channels, 2004
In order to evaluate the methylotrophic yeast Pichia pastoris as means for high-yield production of homogenous D(2S) receptor protein, we have expressed the unmodified D(2S) receptor and various D(2S) receptor fusion constructs under the transcriptional control of the highly inducible promotor of the P. pastoris alcoholoxidase 1 gene in strain SMD1163.
S. Grünewald, W. Haase, E. Molsberger, H. Michel, H. Reiländer   +4 more
semanticscholar   +4 more sources

Metabolic Engineering of Pichia pastoris for High-Level Production of Lycopene.

ACS Synthetic Biology, 2023
Lycopene is widely used in cosmetics, food, and nutritional supplements. Microbial production of lycopene has been intensively studied. However, few metabolic engineering studies on Pichia pastoris have been aimed at achieving high-yield lycopene ...
Xinying Zhang, Shuting Chen, Ying-xiang Lin, Wenjie Li, Denggang Wang, Shupeng Ruan, Yuxin Yang, Shuli Liang   +7 more
semanticscholar   +1 more source

High-level Secretory Production of Leghemoglobin in Pichia pastoris through Enhanced Globin Expression and Heme Biosynthesis.

Bioresource Technology, 2022
Soy leghemoglobin is a key food additive that imparts meaty flavor and color to meat analogs. Here, a Pichia pastoris strain capable of high-yield secretory production of functional leghemoglobin was developed through gene dosage optimization and heme ...
Youran Shao, Changlu Xue, Wen-quan Liu, Siqi Zuo, Pei-lian Wei, Lei Huang, J. Lian, Zhinan Xu   +7 more
semanticscholar   +1 more source

Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9.

ACS Synthetic Biology, 2022
Pichia pastoris, an important methylotrophic yeast, is currently mainly used for the expression of recombinant proteins and has great potential applications in the production of value-added compounds (e.g., chemical and natural products).
Jucan Gao, Junhao Xu, Yimeng Zuo, Cuifang Ye, Leijie Jiang, Linjuan Feng, Lei Huang, Zhinan Xu, J. Lian   +8 more
semanticscholar   +1 more source

Effect of Overexpression of the HAC1 Genes from Pichia pastoris and Saccharomyces cerevisiae on the Heterologous Phytase and Xylanase Production by P. pastoris

Biotekhnologiya, 2019
Effect of overexpression of the HAC1 genes from Pichia pastoris and Saccharomyces cerevisiae on the production of heterologous enzymes, Escherichia coli phytase and Paenibacillus brasilensis xylanase, in P. pastoris cells has been studied. Codon composition of the phytase and xylanase encoding genes was optimized, and the genes were expressed in P ...
L.N. Borshchevskaya, A.N. Kalinina, S.P. Sineoky, M.D. Kashirskaya   +3 more
openaire   +1 more source

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms.

ACS Synthetic Biology, 2022
There is a growing interest in establishing the methylotrophic yeast Pichia pastoris as microbial cell factories for producing fuels, chemicals, and natural products, particularly with methanol as the feedstock.
Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, J. Lian   +5 more
semanticscholar   +1 more source

Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris

Biotechnology Letters, 2013
The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity
Shuli, Liang, Chengjuan, Zou, Ying, Lin, Xuanwei, Zhang, Yanrui, Ye   +4 more
openaire   +2 more sources

Recombinant human serum amyloid P component from Pichia pastoris: production and characterization

Protein Expression and Purification, 2004
Human serum amyloid P component (SAP) was expressed in the methylotrophic yeast Pichia pastoris. SAP cDNA was placed under control of regulatory sequences derived from the alcohol oxidase gene (AOX1), and its protein product was secreted using the Saccharomyces cerevisiae alpha-mating factor signal sequence.
Boysen, S., Fogh-Schultz, B., Andersen, I., Højrup, Peter; id_orcid 0000-0002-7838-6180, Iversen, Jens Jørgen Lønsmann, Wittenhagen, P., Nielsen, E.H., Svehag, Svend-Erik   +7 more
openaire   +3 more sources

Characterization of the house dust mite allergen Der p 21 produced in Pichia pastoris

Protein Expression and Purification, 2014
The development of recombinant house dust mite (HDM) allergens opened the way for the in-depth characterization of these molecules but also provided new opportunities to refine the diagnostic procedures of HDM allergy as well as the allergen-specific immunotherapy through tailor-made treatments.In the present study, the HDM allergen Der p 21 was ...
Pinya, Pulsawat, Montalee, Theeraapisakkun, Emmanuel, Nony, Maxime, Le Mignon, Karine, Jain, Arun, Buaklin, Jongkonnee, Wongpiyabovorn, Kiat, Ruxrungtham, Alain, Jacquet   +8 more
openaire   +2 more sources

Key regulatory elements of a strong constitutive promoter, P GCW14 , from Pichia pastoris

Biotechnology Letters, 2013
The promoter of the Pichia pastoris gene GCW14 is strong and constitutive when glycerol is the available carbon source. To identify the cis-acting elements of this promoter (P GCW14), we constructed expression plasmids where the enhanced green fluorescent protein gene was fused to a series of mutants of P GCW14. We identified one negative (-114 to -94)
Xuanwei, Zhang, Xiuqin, Zhang, Shuli, Liang, Yanrui, Ye, Ying, Lin   +4 more
openaire   +2 more sources