Results 161 to 170 of about 20,634,842 (205)

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Yeast, 2010
AbstractThe yeast vectors described, pYEV and pYEVB, were designed for parallel polymerase chain reaction (PCR) cloning of open reading frames (ORFs) and immediate protein expression in Pichia pastoris. The pYEV vector was used to clone PCR fragments obtained by using Taq or similar polymerase mixes, which leave an A‐base overhang.
Ben, Rao, Xing, Zhong, Yaping, Wang, Qiong, Wu, Zhengbing, Jiang, Lixin, Ma   +5 more
openaire   +2 more sources

Revealing the Structure and Function of P. Pastoris Metabolic Network Using Petri Nets

2015 14th International Symposium on Distributed Computing and Applications for Business Engineering and Science (DCABES), 2015
Due to the limitations of detailed experimental data for large scale metabolic networks, structural analysis of these networks is a growing research topic in bioinformatics field. Among several available structure-oriented approaches, Petri nets has proved to be particular efficient tool, and thus is employed for studying P.
Yufang Wang, Dewu Ding
openaire   +1 more source

Establishing of an equivalent process platform for P. pastoris fermentation in different scales

2018
Die Skalierbarkeit von Prozessen ist in vielen Sektoren sowie Technologien eine Herausforderung. Durch die komplexität der Arbeit mit lebenden Organismen sind biotechnologische Anwendungen keine Ausnahme, welche zeitintensive sowie finanzielle Folgen haben können. Diese Arbeit fokosiert sich auf den Scale-up einer P. pastoris-Fermentation vom 5L Labor-
openaire   +1 more source

Biosynthesis of catharanthine in engineered Pichia pastoris

Nature Synthesis, 2023
Jucan Gao, Yimeng Zuo, Feng Xiao, Yiling Wang, Dongfang Li, Junhao Xu, Cuifang Ye, Linjuan Feng, Leijie Jiang, Tengfei Liu, Di Gao, Bing Ma, Lei Huang, Zhinan Xu, J. Lian   +14 more
semanticscholar   +1 more source

Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions.

Fungal Genetics and Biology, 2016
Tamara Wriessnegger, S. Moser, Anita Emmerstorfer-Augustin, E. Leitner, M. Müller, I. Kaluzna, Martin Schürmann, D. Mink, H. Pichler   +8 more
semanticscholar   +1 more source

A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production

Molecular Biotechnology, 2023
Vijay Elakkya Vijayakumar, Krishnan Venkataraman   +1 more
semanticscholar   +1 more source

Optimization of heterologous expression of the phytase (PPHY) of Pichia anomala in P. pastoris and its applicability in fractionating allergenic glycinin from soy protein

Journal of Industrial Microbiology & Biotechnology, 2014
S. Joshi, T. Satyanarayana
semanticscholar   +1 more source

Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system

World Journal of Microbiology & Biotechnology, 2023
A. Eskandari, Nima Ghahremani Nezhad, T. Leow, Mohd Basyaruddin Abdul Rahman, Siti Nurbaya Oslan   +4 more
semanticscholar   +1 more source

Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris.

Journal of Biotechnology, 2006
Chiawei W. Tsai, Peter F Duggan, R. Shimp, L. Miller, D. Narum   +4 more
semanticscholar   +1 more source

Effects of Temperature and pH on Recombinant Thaumatin II Production by Pichia pastoris

Foods, 2022
Jan Van Impe, Simen Akkermans, Jewel Ann Joseph   +2 more
exaly