Results 181 to 190 of about 23,995,070 (219)

Key regulatory elements of a strong constitutive promoter, P GCW14 , from Pichia pastoris

Biotechnology Letters, 2013
The promoter of the Pichia pastoris gene GCW14 is strong and constitutive when glycerol is the available carbon source. To identify the cis-acting elements of this promoter (P GCW14), we constructed expression plasmids where the enhanced green fluorescent protein gene was fused to a series of mutants of P GCW14. We identified one negative (-114 to -94)
Xuanwei, Zhang, Xiuqin, Zhang, Shuli, Liang, Yanrui, Ye, Ying, Lin   +4 more
openaire   +2 more sources

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Yeast, 2010
AbstractThe yeast vectors described, pYEV and pYEVB, were designed for parallel polymerase chain reaction (PCR) cloning of open reading frames (ORFs) and immediate protein expression in Pichia pastoris. The pYEV vector was used to clone PCR fragments obtained by using Taq or similar polymerase mixes, which leave an A‐base overhang.
Ben, Rao, Xing, Zhong, Yaping, Wang, Qiong, Wu, Zhengbing, Jiang, Lixin, Ma   +5 more
openaire   +2 more sources

Revealing the Structure and Function of P. Pastoris Metabolic Network Using Petri Nets

2015 14th International Symposium on Distributed Computing and Applications for Business Engineering and Science (DCABES), 2015
Due to the limitations of detailed experimental data for large scale metabolic networks, structural analysis of these networks is a growing research topic in bioinformatics field. Among several available structure-oriented approaches, Petri nets has proved to be particular efficient tool, and thus is employed for studying P.
Yufang Wang, Dewu Ding
openaire   +1 more source

Establishing of an equivalent process platform for P. pastoris fermentation in different scales

2018
Die Skalierbarkeit von Prozessen ist in vielen Sektoren sowie Technologien eine Herausforderung. Durch die komplexität der Arbeit mit lebenden Organismen sind biotechnologische Anwendungen keine Ausnahme, welche zeitintensive sowie finanzielle Folgen haben können. Diese Arbeit fokosiert sich auf den Scale-up einer P. pastoris-Fermentation vom 5L Labor-
openaire   +1 more source

Bioconversion of C1 feedstocks for chemical production using Pichia pastoris

Trends in Biotechnology, 2023
Fengxue Xin, Wenming Zhang
exaly  

Methylotrophy of Pichia pastoris: Current Advances, Applications, and Future Perspectives for Methanol-Based Biomanufacturing

ACS Sustainable Chemistry and Engineering, 2022
Kequan Chen, Xin Wang
exaly  

Effects of Temperature and pH on Recombinant Thaumatin II Production by Pichia pastoris

Foods, 2022
Jan van Impe, Simen Akkermans, Jewel Ann Joseph   +2 more
exaly  

Structural and biochemical analyses of the nucleosome containing Komagataella pastoris histones

Journal of Biochemistry, 2022
Tomoya Kujirai, Hitoshi Kurumizaka, Shun-Ichi Sekine   +2 more
exaly  

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Journal of Cellular Physiology, 2020
S A Rahim Rezaee, Mohsen Karbalaei, Hadi Farsiani   +2 more
exaly  

Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae - T1 lipase expression in P. Pastoris

4th Annual International Conference on Advances in Biotechnology (BioTech 2014), 2014
Siti Nurbaya Oslan, Abu Bakar Salleh, Raja Noor Zaliha Raja Abd. Rahman, Thean Chor Leow, Mahiran Basri   +4 more
openaire   +1 more source