Results 181 to 190 of about 47,430 (223)

Efficient precious metal Rh(III) adsorption by waste P. pastoris and P. pastoris surface display from high-density culture

Journal of Hazardous Materials, 2022
Rhodium is one of the most used precious metals in catalysis both in laboratory reactions and industrial processes. However, the adsorption of Rh(III) by microorganisms has been seldomly reported. In this work, waste P. pastoris and recombinant P. pastoris with surface-displayed Rh metal peptides (P.
Minjie, Gao, Yuchen, Zhou, Jiajun, Yan, Li, Zhu, Zhitao, Li, Xiuyu, Hu, Xiaobei, Zhan   +6 more
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Membrane Protein Production in the Yeast P. pastoris

2022
The first crystal structures of recombinant mammalian membrane proteins were solved using high-quality protein that had been produced in yeast cells. One of these, the rat Kv1.2 voltage-gated potassium channel, was synthesized in Pichia pastoris.
Hoor, Ayub, Michelle, Clare, Luke, Broadbent, John, Simms, Alan D, Goddard, Alice J, Rothnie, Roslyn M, Bill   +6 more
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The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris

Gene, 2004
The methylotrophic yeast Pichia pastoris is a popular host for the production of a variety of recombinant proteins. We describe the use of a novel selectable marker, the P. pastoris formaldehyde dehydrogenase gene (FLD1) for DNA-mediated transformations of this yeast. The product of the FLD1 gene (Fld1p) is required for growth of P.
Anthony Jay, Sunga, James M, Cregg
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Recombinant human serum amyloid P component from Pichia pastoris: production and characterization

Protein Expression and Purification, 2004
Human serum amyloid P component (SAP) was expressed in the methylotrophic yeast Pichia pastoris. SAP cDNA was placed under control of regulatory sequences derived from the alcohol oxidase gene (AOX1), and its protein product was secreted using the Saccharomyces cerevisiae alpha-mating factor signal sequence.
Boysen, S., Fogh-Schultz, B., Andersen, I., Højrup, Peter, Iversen, Jens Jørgen Lønsmann, Wittenhagen, P., Nielsen, E.H., Svehag, Svend-Erik   +7 more
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Production of the Human D_2SReceptor in the Methylotrophic Yeast P. pastoris

Receptors and Channels, 2004
In order to evaluate the methylotrophic yeast Pichia pastoris as means for high-yield production of homogenous D(2S) receptor protein, we have expressed the unmodified D(2S) receptor and various D(2S) receptor fusion constructs under the transcriptional control of the highly inducible promotor of the P. pastoris alcoholoxidase 1 gene in strain SMD1163.
Grünewald, S., Haase, W., Molsberger, E., Michel, H. ; https://orcid.org/0000-0003-4351-7846, Reiländer, H.   +4 more
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Key regulatory elements of a strong constitutive promoter, P GCW14 , from Pichia pastoris

Biotechnology Letters, 2013
The promoter of the Pichia pastoris gene GCW14 is strong and constitutive when glycerol is the available carbon source. To identify the cis-acting elements of this promoter (P GCW14), we constructed expression plasmids where the enhanced green fluorescent protein gene was fused to a series of mutants of P GCW14. We identified one negative (-114 to -94)
Xuanwei, Zhang, Xiuqin, Zhang, Shuli, Liang, Yanrui, Ye, Ying, Lin   +4 more
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Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene

Veterinary Microbiology, 2012
The probiotic properties of Pichia pastoris and of a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene were evaluated in broilers. One-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials.
João Rodrigo, Gil de los Santos, Otávio Brod, Storch, Cristina Gevehr, Fernandes, Carlos, Gil-Turnes   +3 more
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Characterization of the house dust mite allergen Der p 21 produced in Pichia pastoris

Protein Expression and Purification, 2014
The development of recombinant house dust mite (HDM) allergens opened the way for the in-depth characterization of these molecules but also provided new opportunities to refine the diagnostic procedures of HDM allergy as well as the allergen-specific immunotherapy through tailor-made treatments.In the present study, the HDM allergen Der p 21 was ...
Pinya, Pulsawat, Montalee, Theeraapisakkun, Emmanuel, Nony, Maxime, Le Mignon, Karine, Jain, Arun, Buaklin, Jongkonnee, Wongpiyabovorn, Kiat, Ruxrungtham, Alain, Jacquet   +8 more
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Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris

Biotechnology Letters, 2013
The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity
Shuli, Liang, Chengjuan, Zou, Ying, Lin, Xuanwei, Zhang, Yanrui, Ye   +4 more
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Revealing the Structure and Function of P. Pastoris Metabolic Network Using Petri Nets

2015 14th International Symposium on Distributed Computing and Applications for Business Engineering and Science (DCABES), 2015
Due to the limitations of detailed experimental data for large scale metabolic networks, structural analysis of these networks is a growing research topic in bioinformatics field. Among several available structure-oriented approaches, Petri nets has proved to be particular efficient tool, and thus is employed for studying P.
Yufang Wang, Dewu Ding
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