Recombinant protein expression in Acanthamoeba castellanii [PDF]
Frontiers in Bioengineering and BiotechnologyThe ongoing quest to improve protein production efficiency, quality, and versatility fuels the exploration of novel expression systems. In this research, we explored the potential of the axenically culturable Acanthamoeba as an alternative for producing ...
Pooja Salunke +4 more
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Special Issue: Recombinant Protein Expression in Microorganisms [PDF]
Microorganisms, 2019 Microorganisms are widely used in industrial biotechnology as cell factories for the sustainable production of a wide range of compounds and chemicals [...]
Trygve Brautaset, Svein Valla
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Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li +4 more
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Recombinant protein expression in microbial systems [PDF]
Frontiers in Microbiology, 2014 With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively.
Germán Leandro Rosano +1 more
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Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]
Scientific ReportsTo meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu +6 more
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Recombinant protein expression in biofilms. [PDF]
AIMS Microbiol, 2019 Biofilm research is usually focused on the prevention or control of biofilm formation. Recently, the significance of the biofilm mode of growth in biotechnological applications received increased attention. Since biofilm reactors show many advantages over suspended cell reactors, especially in their higher biomass density and operational stability ...
Soares A +3 more
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A novel stationary phase approach for improved recombinant protein expression in Escherichia coli: beyond the exponential phase [PDF]
Microbial Cell FactoriesBackground Escherichia coli is a well-known host species for studies on recombinant protein expression, typically induced during the exponential phase.
Yoon-Hyeok Kang +7 more
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Incorporation of regulatory DNA elements within a viral vector improves recombinant protein expression in plants [PDF]
Scientific ReportsPlants have significant potential as recombinant protein expression chassis, as they can produce complex post-translationally modified proteins that are unobtainable using prokaryotic production systems, with almost limitless scalability and ...
Ryan J. Coates +2 more
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Ramping Recombinant Protein Expression in Bacteria. [PDF]
Biochemistry, 2020 Zahurancik WJ +3 more
europepmc +4 more sources
Frontiers in Bioengineering and Biotechnology, 2022 Chinese hamster ovary (CHO) cells are currently the most widely used host cells for recombinant therapeutic protein (RTP) production. Currently, the RTP yields need to increase further to meet the market needs and reduce costs.
Qin Li +8 more
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