Frontiers in Bioengineering and Biotechnology, 2022 Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li +7 more
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Genome‐Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis [PDF]
Advanced ScienceBacillus subtilis is an industrially important microorganism that is often used as a microbial cell factory for the production of recombinant proteins due to its food safety, rapid growth, and powerful secretory capacity.
Xuyang Zhu, Hui Luo, Xinrui Yu
exaly +3 more sources
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells. [PDF]
Protein Expr Purif, 2021 Generating recombinant proteins in insect cells has been made possible via the use of the Baculovirus Expression Vector System (BEVS). Despite the success of many proteins via this platform, some targets remain a challenge due to issues such as ...
Grose C, Putman Z, Esposito D.
europepmc +2 more sources
Choice of selectable marker affects recombinant protein expression in cells and exosomes. [PDF]
J Biol Chem, 2021 Transgenic mammalian cells are used for numerous research, pharmaceutical, industrial, and clinical purposes, and dominant selectable markers are often used to enable the selection of transgenic cell lines.
Guo C +4 more
europepmc +2 more sources
Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li +4 more
doaj +2 more sources
Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges. [PDF]
FEMS Yeast ResKomagataella phaffii has gained recognition as a versatile platform for recombinant protein production, with applications covering biopharmaceuticals, industrial enzymes, food additives, etc. Its advantages include high-level protein expression, moderate
Lv W, Cai M.
europepmc +2 more sources
Recombinant protein expression in Escherichia coli: advances and challenges
Frontiers in Microbiology, 2014 Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
German L Rosano +2 more
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Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion. [PDF]
Biotechnol Bioeng, 2020 We report the scalable production of recombinant proteins in Escherichia coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion in the stationary phase. The method, reliant on engineered strains and plasmids, enables improved
Menacho-Melgar R +7 more
europepmc +2 more sources
Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]
Scientific ReportsTo meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu +6 more
doaj +2 more sources
Polyionic Tags as Enhancers of Protein Solubility in Recombinant Protein Expression
Microorganisms, 2018 Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground.
Vasiliki Paraskevopoulou +1 more
exaly +2 more sources