Results 41 to 50 of about 2,765,447 (281)

Mycobacterium tuberculosis antigen 85B and ESAT-6 expressed as a recombinant fusion protein in Mycobacterium smegmatis elicits cell-mediated immune response in a murine vaccination model [PDF]

, 2013
This is the post-print version of the final paper published in Molecular Immunology. The published article is available from the link below. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting ...
Kishore, U, Leiva, S, Robertson, BD, Rosenkrands, I, Nagy, J, Tsolaki, AG   +5 more
core   +1 more source

Infectious Recombinant Senecavirus A Expressing p16INK4A Protein

International Journal of Molecular Sciences, 2023
Senecavirus A (SVA) is an oncolytic RNA virus, and it is the ideal oncolytic virus that can be genetically engineered for editing. However, there has not been much exploration into creating SVA viruses that carry antitumor genes to increase their oncolytic potential.
Wencheng Gong, Xiaoya Zhao, Xiaoyu Tang, Long Gao, Yuan Sun, Jingyun Ma   +5 more
openaire   +2 more sources

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source

Design and Recombinant Expression of a multiepitope Vaccine Candidate Against Pathogenic Species of Shigella

Vaccine Research, 2021
Introduction: Shigellosis is a form of acute intestinal infection and one of the global health problems that occur in human by pathogenic species of Shigella.
Amir Namvar, Abbas Hajizade, Shahram Nazarian, Davoud Sadeghi, Mohammad Reza Akbari, Yousof Tarverdizade   +5 more
doaj   +1 more source

Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems

Scientific Reports, 2022
The silkworm, Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production in B.
Hiroyuki Kajiura, Ken-ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama   +7 more
doaj   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in

BioTechniques, 2017
Cell lines expressing foreign genes have been widely used to produce a variety of recombinant proteins. However, generating recombinant protein–expressing cell lines is usually a lengthy process and the resulting protein expression levels are often ...
Chiu-An Lo, Alexander W. Greben, Brian Edwin Chen   +2 more
doaj   +1 more source

Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants [PDF]

Plant Molecular Biology Reporter, 2013
Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose.
Shah, Kausar Hussain, Almaghrabi, Bachar, Bohlmann, Holger   +2 more
openaire   +2 more sources

Novel and effective screening system for recombinant protein production in CHO cells

Scientific Reports
At present, biopharmaceuticals have received extensive attention from the society, among which recombinant proteins have a good growth trend and a large market share.
Junhe Zhang, Wenwen Yang, Liao Zhang, Wenqing Li, Xi Zhang, Xiaoyin Wang, Tianyun Wang   +6 more
doaj   +1 more source

Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression

Biotechnology Reports, 2018
Highlights • Easy preparation of monoclonal insect cell lines in a serum free environment.• Protocol uses co-cultivation with untreated feeder cells and antibiotic selection.• Monoclonality allows efficient and stable recombinant protein expression ...
J. Zitzmann, C. Schreiber, Joel Eichmann, Roberto Otmar Bilz, D. Salzig, T. Weidner, P. Czermak   +6 more
semanticscholar   +1 more source