Recombinant Expression of Cyclotides Using Expressed Protein Ligation
, 2020 Cyclotides are naturally occurring microproteins (≈30 residues long) present in several families of plants. All cyclotides share a unique head-to-tail circular knotted topology containing three disulfide bridges forming a cystine knot topology. Cyclotides possess high stability to chemical, physical, and biological degradation and have been reported to
Maria Jose, Campbell +2 more
openaire +3 more sources
Bacterial expression and purification of recombinant Plasmodium yoelii circumsporozoite protein
, 1997 We report the expression and purification of recombinant rodent malarialPlasmodium yoeliicircumsporozoite surface protein (PyCSP) inEscherichia coli.To facilitate purification of the recombinant protein, the PyCSP was expressed as an amino-terminal ...
Kang, A.S. +3 more
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Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells [PDF]
, 2007 Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components.
Buriani, Giampaolo +23 more
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Improvement of downstream processing of recombinant proteins by means of genetic engineering
, 1993 Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
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An Effective Recombinant Protein Expression and Purification System in Saccharomyces cerevisiae
Current Protocols in Molecular Biology, 2018 The expression and purification of recombinant proteins using bacterial vectors is a mature and preferred system to obtain folded and stable proteins. However, functional post‐translational protein modifications, such as glycosylation or phosphorylation,
Ying Xie, Xiao Han, Yansong Miao
semanticscholar +1 more source
Synthetic enhancers including TFREs improve transgene expression in CHO cells
HeliyonThe human cytomegalovirus major immediate early gene (CMV) promoter is currently the most preferred promoter for recombinant therapeutic proteins (RTPs) production in CHO cells.
Hui-Ning Liu +6 more
doaj +1 more source
Expression and purification of recombinant human coagulation Factor VII fused to His-Tag through Gateway technology [PDF]
Background. Factor VII is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. Construction, expression and purification of recombinant FVII fused to poly histidin tag through gateway technology ...
عدالتی فتح آباد, مهدی +6 more
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Immunotherapeutic targeting of membrane Hsp70-expressing tumors using recombinant human granzyme B [PDF]
, 2012 Background: We have previously reported that human recombinant granzyme B (grB) mediates apoptosis in membrane heat shock protein 70 (Hsp70)-positive tumor cells in a perforin-independent ...
Axel Walch +39 more
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Adventitious viruses in insect cell lines used for recombinant protein expression
Protein Expression and Purification, 2017 Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation.
C. Geisler, D. Jarvis
semanticscholar +1 more source
Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein [PDF]
, 1996 Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease.
Milon, Alain +13 more
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