Results 31 to 40 of about 2,765,447 (281)

Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones

Frontiers in Bioengineering and Biotechnology, 2022
Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang, Wenwen Yang, Junhe Zhang, Junhe Zhang, Junhe Zhang, Yunxi Xiao, Wenqing Li, Tianyun Wang, Tianyun Wang   +8 more
doaj   +1 more source

Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21

Frontiers in Microbiology, 2021
Recombinant protein production for medical, academic, or industrial applications is essential for our current life. Recombinant proteins are obtained mainly through microbial fermentation, with Escherichia coli being the host most used. In spite of that,
G. Lozano Terol, J. Gallego-Jara, R. A. Sola Martínez, Adrián Martínez Vivancos, M. Cánovas Díaz, T. de Diego Puente   +5 more
semanticscholar   +1 more source

Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells.
Jun-He Zhang, Jun-He Zhang, Jun-He Zhang, Lin-Lin Shan, Fan Liang, Chen-Yang Du, Jing-Jing Li   +6 more
doaj   +1 more source

Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production

Microbial Cell Factories, 2021
Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (P lacUV5 ...
Fei Du, Yun-qi Liu, Ying‐shuang Xu, Zi-Jia Li, Yu-Zhou Wang, Zixu Zhang, Xiaoman Sun   +6 more
semanticscholar   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

Expression and purification of movement protein of Cucumber green mottle mosaic virus using a plant-virus expression system [PDF]

مجله بیوتکنولوژی کشاورزی, 2013
To produce and purify movement protein of Cucumber green mottle mosaic virus (CGMMV MP), a plant viral vector engineered from an in vivo infectious clone of Zucchini yellow mosaic virus (ZYMV) was used.
Seyyed Mohsen Nassaj Hosseini, Masoud Shams Bakhsh, Ali Hatef Salmanian, Shay Doung Ye   +3 more
doaj   +1 more source

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Frontiers in Bioengineering and Biotechnology, 2022
Animal venoms are complex mixtures containing peptides and proteins known as toxins, which are responsible for the deleterious effect of envenomations. Across the animal Kingdom, toxin diversity is enormous, and the ability to understand the biochemical ...
Esperanza Rivera-de-Torre, Charlotte Rimbault, Timothy P. Jenkins, Christoffer V. Sørensen, Anna Damsbo, Natalie J. Saez, Yoan Duhoo, Celeste Menuet Hackney, Lars Ellgaard, Andreas H. Laustsen   +9 more
doaj   +1 more source

Automation of large scale transient protein expression in mammalian cells [PDF]

, 2011
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Susan Daenke, Jones, M, E. Yvonne Jones, Siebold, Christian, Daenke, Susan, Christian Siebold, Bishop, Benjamin, Margaret Jones, Clay, Jordan E, Stuart, David I., A. Radu Aricescu, Zhao, Y, Benjamin Bishop, Aricescu, Alexandru R, Radu Aricescu, A, Stuart, David, Yvonne Jones, E, Weixian Lu, Stuart, David I, Clay, JE, Daenke, S, Aricescu, Alexandru, Bishop, B, Jones, EY, Clay, Jordan E., Yuguang Zhao, Jones, Margaret, Lu, Weixian, David I. Stuart, Radu Aricescu, A., Yvonne Jones, E., Aricescu, AR, Jones, Edith, Jordan E. Clay, Zhao, Yuguang, Jones, E Yvonne, Lu, W, Siebold, C, Stuart, DI   +38 more
core   +1 more source

In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

Indonesian Journal of Biotechnology, 2020
The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV).  The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates ...
Aris Haryanto, Hevi Wihadmadyatami, Nastiti Wijayanti   +2 more
doaj   +1 more source