Results 21 to 30 of about 6,659,682 (289)

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target [PDF]

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

Automation of large scale transient protein expression in mammalian cells [PDF]

, 2011
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Susan Daenke, Jones, M, E. Yvonne Jones, Siebold, Christian, Daenke, Susan, Christian Siebold, Bishop, Benjamin, Margaret Jones, Clay, Jordan E, Stuart, David I., A. Radu Aricescu, Zhao, Y, Benjamin Bishop, Aricescu, Alexandru R, Radu Aricescu, A, Stuart, David, Yvonne Jones, E, Weixian Lu, Stuart, David I, Clay, JE, Daenke, S, Aricescu, Alexandru, Bishop, B, Jones, EY, Clay, Jordan E., Yuguang Zhao, Jones, Margaret, Lu, Weixian, David I. Stuart, Radu Aricescu, A., Yvonne Jones, E., Aricescu, AR, Jones, Edith, Jordan E. Clay, Zhao, Yuguang, Jones, E Yvonne, Lu, W, Siebold, C, Stuart, DI   +38 more
core   +1 more source

Expression and purification of movement protein of Cucumber green mottle mosaic virus using a plant-virus expression system [PDF]

مجله بیوتکنولوژی کشاورزی, 2013
To produce and purify movement protein of Cucumber green mottle mosaic virus (CGMMV MP), a plant viral vector engineered from an in vivo infectious clone of Zucchini yellow mosaic virus (ZYMV) was used.
Seyyed Mohsen Nassaj Hosseini, Masoud Shams Bakhsh, Ali Hatef Salmanian, Shay Doung Ye   +3 more
doaj   +1 more source

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Frontiers in Bioengineering and Biotechnology, 2022
Animal venoms are complex mixtures containing peptides and proteins known as toxins, which are responsible for the deleterious effect of envenomations. Across the animal Kingdom, toxin diversity is enormous, and the ability to understand the biochemical ...
Esperanza Rivera-de-Torre, Charlotte Rimbault, Timothy P. Jenkins, Christoffer V. Sørensen, Anna Damsbo, Natalie J. Saez, Yoan Duhoo, Celeste Menuet Hackney, Lars Ellgaard, Andreas H. Laustsen   +9 more
doaj   +1 more source

Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]

, 1993
Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
core   +1 more source

Mycobacterium tuberculosis antigen 85B and ESAT-6 expressed as a recombinant fusion protein in Mycobacterium smegmatis elicits cell-mediated immune response in a murine vaccination model [PDF]

, 2013
This is the post-print version of the final paper published in Molecular Immunology. The published article is available from the link below. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting ...
Kishore, U, Leiva, S, Robertson, BD, Rosenkrands, I, Nagy, J, Tsolaki, AG   +5 more
core   +1 more source

In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

Indonesian Journal of Biotechnology, 2020
The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV).  The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates ...
Aris Haryanto, Hevi Wihadmadyatami, Nastiti Wijayanti   +2 more
doaj   +1 more source