Results 71 to 80 of about 2,765,447 (281)

Expression, Delivery and Function of Insecticidal Proteins Expressed by Recombinant Baculoviruses [PDF]

Viruses, 2015
Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortening the time required for infection ...
Jeremy Kroemer, Bryony Bonning, Robert Harrison   +2 more
openaire   +4 more sources

Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells

Applied Microbiology and Biotechnology
Abstract Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific
Lu-Lu Yang, Miao Zhang, Jia-Liang Guo, Ming-Ming Han, Le-Le Qiu, Li-Jie Gao, Liu-Su Cui, Xiao-Yin Wang, Tian-Yun Wang, Yan-Long Jia   +9 more
doaj   +1 more source

An efficient protocol to enhance recombinant protein expression using ethanol in Escherichia coli

MethodsX, 2015
Bacterial cells can be engineered to express non-native genes, resulting in the production of, recombinant proteins, which have various biotechnological and pharmaceutical applications.
Gaurav Chhetri, Parismita Kalita, Timir Tripathi   +2 more
doaj   +1 more source

Tunable recombinant protein expression in E. coli: enabler for continuous processing?

Applied Microbiology and Biotechnology, 2016
Tuning of transcription is a powerful process technological tool for efficient recombinant protein production in Escherichia coli. Many challenges such as product toxicity, formation of inclusion bodies, cell death, and metabolic burden are associated ...
Lukas Marschall, P. Sagmeister, C. Herwig   +2 more
semanticscholar   +1 more source

Conversion of bacterially expressed recombinant prion protein [PDF]

Methods, 2011
The infectivity associated with prion disease sets it apart from a large group of late-onset neurodegenerative disorders that shares the characteristics of protein aggregation and neurodegeneration. The unconventional infectious agent, PrP(Sc), is an aberrantly folded form of the normal prion protein (PrP(C)) and the PrP(C)-to-PrP(Sc) conversion is a ...
Fei, Wang, Xinhe, Wang, Jiyan, Ma
openaire   +2 more sources

Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification

Frontiers in Microbiology, 2018
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for ...
Claudia Ortega, Claudia Ortega, Daniel Prieto, Daniel Prieto, Cecilia Abreu, Cecilia Abreu, Pablo Oppezzo, Pablo Oppezzo, Agustín Correa, Agustín Correa   +9 more
doaj   +1 more source

Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells

PLoS ONE, 2016
The Baculovirus Expression Vector System (BEVS) is widely used to produce high amounts of recombinant proteins. Nevertheless, generating recombinant baculovirus in high quality is rather time-consuming and labor-intensive.
M. Bleckmann, Margitta Schürig, Fang-Fang Chen, Zen-Zen Yen, N. Lindemann, Steffen Meyer, Johannes Spehr, J. van den Heuvel   +7 more
semanticscholar   +1 more source

Expression of recombinant proteins in insect and mammalian cells [PDF]

Methods, 2018
Purified recombinant proteins are key reagents in academic and industrial research. The ability to make these proteins quickly often relies on the availability of higher eukaryotic cell hosts such as insect and mammalian cells where there is a very wide range of post-translational modifications, protein folding and trafficking pathways.
Mckenzie, Edward, Abbott, WM
openaire   +3 more sources

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing

AMB Express
Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges.
Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +12 more
doaj   +1 more source

Expression of fluorescent tagged recombinant erythroferrone protein

Asian Pacific Journal of Tropical Biomedicine, 2018
Objective: To produce fluorescent tagged recombinant erythroferrone protein (ERFE_eGFP) for laboratory investigations. Methods: Erythroferrone (ERFE) gene was fused to green fluorescent protein (eGFP) gene and cloned in a pSecTag2Hygro plasmid.
Min Min Than, Jetsada Ruangsuriya, Chairat Uthaipibull, Somdet Srichairatanakool   +3 more
doaj   +1 more source