Results 91 to 100 of about 1,066,221 (297)

Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. Identification of the crucial role of a threonine residue for enzyme activity [PDF]

, 2005
Volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (AAT). We demonstrate here that, in Charentais melon (Cucumis melo var.
A. Aggelis, A. Aharoni, A.-D. Bauchot, A.G. Perez, A.M. Dandekar, Alain Latché, B. St Pierre, B. St Pierre, B.G. Defilippi, B.G. Defilippi, C. Aubert, C. Sanz, Daniel Manríquez, E. Pichersky, F. Flores, F. Jeanmougin, F. Yahyaoui, Farid Regad, Francisco B. Flores, H. Shiota, H. Yoshimoto, I. Zabetakis, Islam El-Sharkawy, J. Beekwilder, J. D’Auria, J. Song, J.C. Beaulieu, J.P. Mattheis, Jean-Claude Pech, M. Guis, M. Harada, M. Shalit, M. Shalit, M.M. Bradford, Mondher Bouzayen, N. Dudareva, N. Dudareva, N. Dudareva, N.A. Mir, N.M.M. Paillard, P.K. Boss, R. Ayub, R. Medina-Suarez, R.D.M. Page, R.M. Beaudry, S. Elss, S. Lurie, S.F. Altschul, S.G. Wyllie, V. Homatidou, X. Fan, X. Ma, Y. Wang, Y.W. Nam   +53 more
core   +2 more sources

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Optimization of a novel expression system for recombinant protein production in CHO cells

Scientific Reports
Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered.
Junhe Zhang, Chenyang Du, Yue Pan, Zhan Zhang, Ruoyuan Feng, Mengyao Ma, Tianyun Wang   +6 more
doaj   +1 more source

Characterization of HSP90 isoforms in transformed bovine leukocytes infected with Theileria annulata [PDF]

, 2016
HSP90 chaperones are essential regulators of cellular function, as they ensure the appropriate conformation of multiple key client proteins. Four HSP90 isoforms were identified in the protozoan parasite Theileria annulata.
Calder, Ewen D.D., Devaney, Eileen, Gillan, Victoria, Hostettler, Isabel, Kinnaird, Jane H., Shiels, Brian R., Singh, Meetali, Tatu, Utpal, Weir, William   +8 more
core   +2 more sources

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Retargeted adenoviruses for radiation-guided gene delivery [PDF]

, 2016
The combination of radiation with radiosensitizing gene delivery or oncolytic viruses promises to provide an advantage that could improve the therapeutic results for glioblastoma. X-rays can induce significant molecular changes in cancer cells.
A Beetz, AP Patel, BL Davidson, C Roller, Cancer Genome Atlas Research Network, D E Hallahan, DA Boothman, DA Reardon, DA Spencer, DE Hallahan, DY Dadey, F Tameire, G Bajocchi, H Hsu, H Li, H Yan, HO McCarthy, JJ Mezhir, K Daino, L N Kaliberova, LM Hendershot, M Gonzalez-Gronow, M Hamdi, M Martin, M Westphal, MA Arap, MJ Gray, N Belousova, N Mittereder, R Datta, R Stupp, R Stupp, RJ Passarella, RR Weichselbaum, S A Kaliberov, S Akli, S Mittal, S Parisi, S Takahashi, SA Kaliberov, SC Noureddini, SL Eck, SS Talibi, T Ichikawa, TA Ajith, TC He, Ulasov IV, V Kapoor, VN Krasnykh, WL Blalock, WW Zhang, X Xing, Y Touchefeu, Y Zhang, YL Tsai   +54 more
core   +2 more sources

Data Mining for Expressivity of Recombinant Protein Expression

Transactions of the Japanese Society for Artificial Intelligence, 2006
We analyzed the expressivity of recombinant proteins by using data mining methods. The expression technique of recombinant protein is a key step towards elucidating the functions of genes discovered through genomic sequence projects. We have studied the productive efficiency of recombinant proteins in fission yeast, Schizosaccharomyces pombe (S.pombe),
Satoshi KIRA, Atsushi ISOAI, Masayuki YAMAMURA   +2 more
openaire   +2 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Controlling sap-sucking insect pests with recombinant endophytes expressing plant lectin [PDF]

, 2010
We developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins. Fungal endophyte of Chaetomium globosum YY-11 with anti-fungi activities was isolated from rape seedlings, and bacterial endophytes of SJ-10 ...
Gaofu Qi, Nan Lan, Xianfang Zhang, Xinrong Ma, Xiuyun Zhao   +4 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source