Results 111 to 120 of about 2,765,447 (281)

Introduction of recombinant antigens against Jembrana Disease Virus into plants

open access: yes, 2010
Jembrana disease virus (JDV) is a bovine lentivirus that causes an acute severe disease syndrome in banteng cattle (Bos javanicus) in Indonesia. In order to develop a vaccine, JDV capsid (CA) protein together with transactivator of transcription (Tat ...
Singh, Meenu
core  

Recombinant Protein Expression in Baculovirus-Infected Insect Cells

open access: yesMethods in Enzymology, 2014
The purpose of this protocol is to enable the user to produce a recombinant protein using the baculovirus-insect cell expression system.
D. Jarvis
semanticscholar   +1 more source

A new Gateway vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis

open access: yes, 2007
A major obstacle associated with recombinant protein over-expression in Escherichia coli is the production of insoluble inclusion bodies, a problem particularly pronounced with Mycobacterium tuberculosis proteins.
Moreland, NJ   +4 more
core   +1 more source

Expression, Purfication and endotoxin removal of Brucella melitensis DnaK and Omp31 proteins

open access: yesIranian South Medical Journal, 2014
Background: New strategies are needed to protect against Brucella melitensis infection. Subunit vaccines offer a promising approach because they can stimulate both cellular and humoral immunity, so high production of recombinant protein with less content
Amir Ghasemi   +3 more
doaj  

Generation of a vector suite for protein solubility screening

open access: yesFrontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea   +5 more
doaj   +1 more source

Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells

open access: yes, 2002
East Coast fever (ECF) is a fatal disease of cattle caused by the protozoan parasite Theileria parva. The development of a subunit vaccine, based on the sporozoite-specific surface antigen p67, has been hampered by difficulties in achieving high-level ...
Kaba, S.A.   +4 more
core  

GlycoDelete technology: simplifying mammalian cell N-glycosylation for recombinant protein expression

open access: yesNature Biotechnology, 2014
Heterogeneity in the N-glycans on therapeutic proteins causes difficulties for protein purification and process reproducibility and can lead to variable therapeutic efficacy.
L. Meuris   +15 more
semanticscholar   +1 more source

Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system

open access: gold, 2021
Julie Harnischfeger   +6 more
openalex   +1 more source

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