Results 101 to 110 of about 2,765,447 (281)

Genome engineering for improved recombinant protein expression in Escherichia coli

Microbial Cell Factories, 2014
A metabolic engineering perspective which views recombinant protein expression as a multistep pathway allows us to move beyond vector design and identify the downstream rate limiting steps in expression.
Shubhashree Mahalik, Ashish K. Sharma, K. J. Mukherjee   +2 more
semanticscholar   +1 more source

Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome

, 2022
Josie L. McQuillan, Anthony J. Berndt, Ashley E. Sproles, Stephen P. Mayfield, Jagroop Pandhal   +4 more
openalex   +1 more source

Co-expression of Factor VIII with anti-FVIII Camelid antibody ligands: Effect on expression levels of bio-therapeutic FVIII [PDF]

, 2015
Production of recombinant FVIII, the protein that is missing or dysfunctional in haemophilia A patients, is highly inefficient compared to other recombinant clotting factors such as FIX.
Tolley, Caroline
core  

Development of Escherichia coli and Mycobacterium smegmatis recombinants expressing major Mycobacterium tuberculosis-specific antigenic proteins

International Journal of Mycobacteriology, 2016
Objective/background: Mycobacterium tuberculosis is an obligate pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of most tuberculosis (TB) cases. Until today, the only approved TB vaccine is Bacille Calmette Guerin (BCG)
Hanady A Amoudy, Hussain A Safar, Abu S Mustafa   +2 more
doaj   +1 more source

Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli

, 2006
Amelogenin is a dental enamel matrix protein involved in formation of dental enamel. In this study, we have expressed two different recombinant murine amelogenins in Escherichia coli: the untagged rM179, and the histidine tagged rp(H)M180, identical to ...
Andersson, Christer, Bülow, Leif, Lyngstadaas, P, Bülow, Leif,, Svensson Bonde, Johan, Svensson Bonde, Johan,, Reseland, JE, Lund University.   +7 more
core   +1 more source

Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis. [PDF]

, 2012
BACKGROUND: Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) virus of the Reoviridae family, which encodes its genes in ten linear dsRNA segments.
Roy Polly, Boyce, Mark, Roy, Polly, Celma, Cristina CP, Cristina C P Celma, Boyce Mark, Mark Boyce, Polly Roy, Celma Cristina C P   +8 more
core   +1 more source

High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP

, 2022
Mycobacterium tuberculosis membrane protein biochemistry and structural biology studies are often hampered by challenges in protein expression and selection for well-expressing protein candidates, suitable for further investigation.
Bennett, Matthew D.,, Grāve, Kristīne,, Högbom, Martin,   +2 more
core   +1 more source

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Journal of Visualized Experiments, 2014
The expression and purification of large amounts of recombinant protein complexes is an essential requirement for structural biology studies. For over two decades, prokaryotic expression systems such as E.
N. Portolano, P. Watson, L. Fairall, C. J. Millard, Charles P. Milano, Yun Song, S. Cowley, J. Schwabe   +7 more
semanticscholar   +1 more source

Recombinant expression of fungal oxidases for industrial application

, 2005
Laccases catalyse the oxidation of a range of organic substrates coupled to the reduction of molecular oxygen to water. They are members of the ubiquitous blue multi-copper oxidase family.
Piscitelli, Alessandra
core  

Environment control to improve recombinant protein yields in plants based on Agrobacterium-mediated transient gene expression

Frontiers in Bioengineering and Biotechnology, 2016
Agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. To achieve economically feasible upstream production and downstream processing, two yield parameters should be ...
Naomichi eFujiuchi, Nobuyuki eMatoba, Nobuyuki eMatoba, Ryo eMatsuda   +3 more
doaj   +1 more source