Results 61 to 70 of about 2,765,447 (281)
Synthetic and Systems BiotechnologyChinese hamster ovary (CHO) cells undergo endoplasmic reticulum stress (ERS) during intensive recombinant protein production, triggering the unfolded protein response (UPR) to balance cell survival and protein output.
Qi Zhao +14 more
doaj +1 more source
BIO Web of Conferences, 2020 Newcastle Disease Virus (NDV) is an infectious disease that infect many kinds of wild and domesticated birds. Infection of NDV become a massive problem for poultry industry around the world especially in Indonesia. Vaccination is an effort to prevent the
Pandu Satria Wiratama Ahmad +1 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for
Arshpreet Bhatwa +5 more
semanticscholar +1 more source
Frontiers in Plant SciencePlant microRNAs (miRNAs) are small non-coding RNA molecules that usually negatively regulate gene expression at the post-transcriptional level. Recent data reveal that plant miRNAs are not limited to individual plants but can transfer across different ...
Lei Shi +7 more
doaj +1 more source
A novel methanol-free Pichia pastoris system for recombinant protein expression
Microbial Cell Factories, 2016 BackgroundAs one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (PAOX1) which is strongly induced by methanol.
W. Shen +9 more
semanticscholar +1 more source
Cloning and Expression of Leptospira LipL32 Antigen as a Candidate for Rapid Diagnosis [PDF]
Journal of Fasa University of Medical Sciences, 2013 Background and Objective: Leptospirosis as an important emerging infectious zoonotic disease caused by spirochetes of the genus Leptospira. Given the low sensitivity and long duration of its culture, the diagnosis of leptospirosis is mainly based on ...
Nooshin Sohrabi, Shohreh Azimi
doaj +1 more source
Signal peptides for recombinant protein secretion in bacterial expression systems
Microbial Cell Factories, 2018 The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into the culture supernatant of a bacterial expression host greatly facilitates their downstream processing and significantly reduces the production costs.
R. Freudl
semanticscholar +1 more source
The roles of histone methylation in the regulation of abiotic stress responses in plants
Plant StressAbiotic stress greatly threaten plants’ growth and ultimately impact crop productivity. Plants have evolved sophisticated regulatory mechanisms to cope with stressful conditions, including undergoing physiological changes through genetic and epigenetic ...
Lei Shi, Xiaoyun Cui, Yuan Shen
doaj +1 more source
Critical reflections on synthetic gene design for recombinant protein expression.
Current Opinion in Structural Biology, 2016 Gene synthesis enables the exploitation of the degeneracy of the genetic code to boost expression of recombinant protein targets for structural studies.
A. Parret, H. Besir, R. Meijers
semanticscholar +1 more source
Data Mining for Expressivity of Recombinant Protein Expression
Transactions of the Japanese Society for Artificial Intelligence, 2006 We analyzed the expressivity of recombinant proteins by using data mining methods. The expression technique of recombinant protein is a key step towards elucidating the functions of genes discovered through genomic sequence projects. We have studied the productive efficiency of recombinant proteins in fission yeast, Schizosaccharomyces pombe (S.pombe),
KIRA, Satoshi +2 more
openaire +2 more sources