Results 191 to 200 of about 47,430 (223)

Effect of Overexpression of the HAC1 Genes from Pichia pastoris and Saccharomyces cerevisiae on the Heterologous Phytase and Xylanase Production by P. pastoris

Biotekhnologiya, 2019
Effect of overexpression of the HAC1 genes from Pichia pastoris and Saccharomyces cerevisiae on the production of heterologous enzymes, Escherichia coli phytase and Paenibacillus brasilensis xylanase, in P. pastoris cells has been studied. Codon composition of the phytase and xylanase encoding genes was optimized, and the genes were expressed in P ...
L.N. Borshchevskaya, A.N. Kalinina, S.P. Sineoky, M.D. Kashirskaya   +3 more
openaire   +1 more source

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Yeast, 2010
AbstractThe yeast vectors described, pYEV and pYEVB, were designed for parallel polymerase chain reaction (PCR) cloning of open reading frames (ORFs) and immediate protein expression in Pichia pastoris. The pYEV vector was used to clone PCR fragments obtained by using Taq or similar polymerase mixes, which leave an A‐base overhang.
Ben, Rao, Xing, Zhong, Yaping, Wang, Qiong, Wu, Zhengbing, Jiang, Lixin, Ma   +5 more
openaire   +2 more sources

Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris

Journal of Biotechnology, 2006
Production of recombinant malaria proteins in the methylotrophic yeast Pichia pastoris has been difficult due to constraints in transcription, translation and/or post-translation controls. Use of codon-optimized genes has resolved many of the transcriptional controls; however, efforts to overcome translational and post-translational modifications ...
Chiawei W, Tsai, Peter F, Duggan, Richard L, Shimp, Louis H, Miller, David L, Narum   +4 more
openaire   +2 more sources

P. Cesare A. de Cara, Gli Hyksôs o Re Pastori di Egitto

2018
Bonner Jahrbücher, Bd.
openaire   +1 more source

Establishing of an equivalent process platform for P. pastoris fermentation in different scales

2018
Die Skalierbarkeit von Prozessen ist in vielen Sektoren sowie Technologien eine Herausforderung. Durch die komplexit t der Arbeit mit lebenden Organismen sind biotechnologische Anwendungen keine Ausnahme, welche zeitintensive sowie finanzielle Folgen haben k nnen. Diese Arbeit fokosiert sich auf den Scale-up einer P.
openaire   +1 more source

Recombinant doubly-deglycosylated Der p 1 expression in Pichia pastoris

Journal of Allergy and Clinical Immunology, 2011
G. Salamanca, H. Ipsen, R. Gutiérrez, R.I. Monsalve   +3 more
openaire   +1 more source

Synthesis of heterologous G protein-coupled receptors in the methylotrophic yeast P. pastoris

Doklady Biochemistry and Biophysics, 2011
A S, Gerasimov, A A, Shul'ga, O A, Zeinalov, K G, Skryabin   +3 more
openaire   +2 more sources

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Journal of Cellular Physiology, 2020
Mohsen Karbalaei, Sa Rahim Rezaee, Hadi Farsiani   +2 more
exaly  

Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis

Advanced Science, 2023
Fei Yu, Xinrui Zhao, Jingwen Zhou
exaly  

Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae - T1 lipase expression in P. Pastoris

4th Annual International Conference on Advances in Biotechnology (BioTech 2014), 2014
Siti Nurbaya Oslan, Abu Bakar Salleh, Raja Noor Zaliha Raja Abd. Rahman, Thean Chor Leow, Mahiran Basri   +4 more
openaire   +1 more source