Results 191 to 200 of about 20,152,363 (248)

Membrane Protein Production in the Yeast P. pastoris.

Methods in molecular biology, 2022
The first crystal structures of recombinant mammalian membrane proteins were solved using high-quality protein that had been produced in yeast cells. One of these, the rat Kv1.2 voltage-gated potassium channel, was synthesized in Pichia pastoris.
Hoor Ayub, Michelle Clare, Luke M. Broadbent, J. Simms, A. Goddard, A. Rothnie, R. Bill   +6 more
semanticscholar   +3 more sources

The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris.

Gene, 2004
The methylotrophic yeast Pichia pastoris is a popular host for the production of a variety of recombinant proteins. We describe the use of a novel selectable marker, the P. pastoris formaldehyde dehydrogenase gene (FLD1) for DNA-mediated transformations of this yeast. The product of the FLD1 gene (Fld1p) is required for growth of P.
Anthony Jay J. Sunga, J. Cregg
semanticscholar   +4 more sources

Recombinant cryptogein production by P. pastoris

New Biotechnology, 2012
Michal Obořil, Kateřina Dadáková, Nikola Ptáčková, T. Kašparovský, J. Lochman   +4 more
semanticscholar   +2 more sources

Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene.

Veterinary Microbiology, 2012
The probiotic properties of Pichia pastoris and of a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene were evaluated in broilers. One-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials.
J. R. Gil de los Santos, O. Storch, C. G. Fernandes, C. Gil-Turnes   +3 more
semanticscholar   +3 more sources

Production of the human D2S receptor in the methylotrophic yeast P. pastoris.

Receptors and Channels, 2004
In order to evaluate the methylotrophic yeast Pichia pastoris as means for high-yield production of homogenous D(2S) receptor protein, we have expressed the unmodified D(2S) receptor and various D(2S) receptor fusion constructs under the transcriptional control of the highly inducible promotor of the P. pastoris alcoholoxidase 1 gene in strain SMD1163.
S. Grünewald, W. Haase, E. Molsberger, H. Michel, H. Reiländer   +4 more
semanticscholar   +4 more sources

Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris.

Journal of Biotechnology, 2006
Production of recombinant malaria proteins in the methylotrophic yeast Pichia pastoris has been difficult due to constraints in transcription, translation and/or post-translation controls. Use of codon-optimized genes has resolved many of the transcriptional controls; however, efforts to overcome translational and post-translational modifications ...
Chiawei W. Tsai, Peter F Duggan, R. Shimp, L. Miller, D. Narum   +4 more
semanticscholar   +3 more sources

Bioconversion of C1 feedstocks for chemical production using Pichia pastoris.

Trends in Biotechnology, 2023
Bioconversion of C1 feedstocks for chemical production offers a promising solution to global challenges such as the energy and food crises and climate change.
Feng Guo, Yangyi Qiao, F. Xin, Wenming Zhang, M. Jiang   +4 more
semanticscholar   +1 more source

Metabolic Engineering of Pichia pastoris for High-Level Production of Lycopene.

ACS Synthetic Biology, 2023
Lycopene is widely used in cosmetics, food, and nutritional supplements. Microbial production of lycopene has been intensively studied. However, few metabolic engineering studies on Pichia pastoris have been aimed at achieving high-yield lycopene ...
Xinying Zhang, Shuting Chen, Ying-xiang Lin, Wenjie Li, Denggang Wang, Shupeng Ruan, Yuxin Yang, Shuli Liang   +7 more
semanticscholar   +1 more source

Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9.

ACS Synthetic Biology, 2022
Pichia pastoris, an important methylotrophic yeast, is currently mainly used for the expression of recombinant proteins and has great potential applications in the production of value-added compounds (e.g., chemical and natural products).
Jucan Gao, Junhao Xu, Yimeng Zuo, Cuifang Ye, Leijie Jiang, Linjuan Feng, Lei Huang, Zhinan Xu, J. Lian   +8 more
semanticscholar   +1 more source

High-level Secretory Production of Leghemoglobin in Pichia pastoris through Enhanced Globin Expression and Heme Biosynthesis.

Bioresource Technology, 2022
Soy leghemoglobin is a key food additive that imparts meaty flavor and color to meat analogs. Here, a Pichia pastoris strain capable of high-yield secretory production of functional leghemoglobin was developed through gene dosage optimization and heme ...
Youran Shao, Changlu Xue, Wen-quan Liu, Siqi Zuo, Pei-lian Wei, Lei Huang, J. Lian, Zhinan Xu   +7 more
semanticscholar   +1 more source