Results 201 to 210 of about 20,152,363 (248)

Effect of Overexpression of the HAC1 Genes from Pichia pastoris and Saccharomyces cerevisiae on the Heterologous Phytase and Xylanase Production by P. pastoris

Biotekhnologiya, 2019
Effect of overexpression of the HAC1 genes from Pichia pastoris and Saccharomyces cerevisiae on the production of heterologous enzymes, Escherichia coli phytase and Paenibacillus brasilensis xylanase, in P. pastoris cells has been studied. Codon composition of the phytase and xylanase encoding genes was optimized, and the genes were expressed in P ...
L.N. Borshchevskaya, A.N. Kalinina, S.P. Sineoky, M.D. Kashirskaya   +3 more
openaire   +1 more source

Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris

Biotechnology Letters, 2013
The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity
Shuli, Liang, Chengjuan, Zou, Ying, Lin, Xuanwei, Zhang, Yanrui, Ye   +4 more
openaire   +2 more sources

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms.

ACS Synthetic Biology, 2022
There is a growing interest in establishing the methylotrophic yeast Pichia pastoris as microbial cell factories for producing fuels, chemicals, and natural products, particularly with methanol as the feedstock.
Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, J. Lian   +5 more
semanticscholar   +1 more source

Recombinant human serum amyloid P component from Pichia pastoris: production and characterization

Protein Expression and Purification, 2004
Human serum amyloid P component (SAP) was expressed in the methylotrophic yeast Pichia pastoris. SAP cDNA was placed under control of regulatory sequences derived from the alcohol oxidase gene (AOX1), and its protein product was secreted using the Saccharomyces cerevisiae alpha-mating factor signal sequence.
Boysen, S., Fogh-Schultz, B., Andersen, I., Højrup, Peter; id_orcid 0000-0002-7838-6180, Iversen, Jens Jørgen Lønsmann, Wittenhagen, P., Nielsen, E.H., Svehag, Svend-Erik   +7 more
openaire   +3 more sources

Characterization of the house dust mite allergen Der p 21 produced in Pichia pastoris

Protein Expression and Purification, 2014
The development of recombinant house dust mite (HDM) allergens opened the way for the in-depth characterization of these molecules but also provided new opportunities to refine the diagnostic procedures of HDM allergy as well as the allergen-specific immunotherapy through tailor-made treatments.In the present study, the HDM allergen Der p 21 was ...
Pinya, Pulsawat, Montalee, Theeraapisakkun, Emmanuel, Nony, Maxime, Le Mignon, Karine, Jain, Arun, Buaklin, Jongkonnee, Wongpiyabovorn, Kiat, Ruxrungtham, Alain, Jacquet   +8 more
openaire   +2 more sources

Key regulatory elements of a strong constitutive promoter, P GCW14 , from Pichia pastoris

Biotechnology Letters, 2013
The promoter of the Pichia pastoris gene GCW14 is strong and constitutive when glycerol is the available carbon source. To identify the cis-acting elements of this promoter (P GCW14), we constructed expression plasmids where the enhanced green fluorescent protein gene was fused to a series of mutants of P GCW14. We identified one negative (-114 to -94)
Xuanwei, Zhang, Xiuqin, Zhang, Shuli, Liang, Yanrui, Ye, Ying, Lin   +4 more
openaire   +2 more sources

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Yeast, 2010
AbstractThe yeast vectors described, pYEV and pYEVB, were designed for parallel polymerase chain reaction (PCR) cloning of open reading frames (ORFs) and immediate protein expression in Pichia pastoris. The pYEV vector was used to clone PCR fragments obtained by using Taq or similar polymerase mixes, which leave an A‐base overhang.
Ben, Rao, Xing, Zhong, Yaping, Wang, Qiong, Wu, Zhengbing, Jiang, Lixin, Ma   +5 more
openaire   +2 more sources

Revealing the Structure and Function of P. Pastoris Metabolic Network Using Petri Nets

2015 14th International Symposium on Distributed Computing and Applications for Business Engineering and Science (DCABES), 2015
Due to the limitations of detailed experimental data for large scale metabolic networks, structural analysis of these networks is a growing research topic in bioinformatics field. Among several available structure-oriented approaches, Petri nets has proved to be particular efficient tool, and thus is employed for studying P.
Yufang Wang, Dewu Ding
openaire   +1 more source

Heterologous expression of FMDV immunodominant epitopes and HSP70 in P. pastoris and the subsequent immune response in mice

Veterinary Microbiology, 2007
Mycobacterium tuberculosis heat shock protein70 (HSP70) is a major antigen with both chaperone and cytokine functions. It has been used as an adjuvant to induce or potentiate humoral and cellular immunity, both in the form of a mixture with peptide antigens, and as a fusion protein.
Chunxia, Su, Xiangguo, Duan, Xiuqing, Wang, Chen, Wang, Rubing, Cao, Bin, Zhou, Puyan, Chen   +6 more
openaire   +2 more sources

A Simple and Rapid Method for Selecting High Producers of Recombinant Proteins in Individual Clones of P. pastoris

Combinatorial Chemistry & High Throughput Screening, 2010
The identification of clones expressing high levels of recombinant protein in Pichia pastoris is usually dependant upon SDS-PAGE, Western blotting, or bioactivity-based assays that are labour and time-consuming. We describe a rapid method that images green fluorescence protein (GFP) of individual P. pastoris clones transformed with vectors that express
Taha H, Al-Samarrai, Christopher A, Kirk, William T, Jones, Dawn, Harvey, Matthew D, Templeton   +4 more
openaire   +2 more sources