Results 91 to 100 of about 19,200,433 (281)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Converting Immune Cold into Hot by Biosynthetic Functional Vesicles to Boost Systematic Antitumor Immunity

iScience, 2020
Summary: Immune cold tumor characterized by low immunogenicity, insufficient and exhausted tumor-infiltrating lymphocytes, and immunosuppressive microenvironment is the main bottleneck responsible for low patient response rate of immune checkpoint ...
Ming Wu, Dongye Zheng, Da Zhang, Peiwen Yu, Lianli Peng, Feng Chen, Ziguo Lin, Zhixiong Cai, Jiong Li, Zuwu Wei, Xinyi Lin, Jingfeng Liu, Xiaolong Liu   +12 more
doaj   +1 more source

On the superlinear Steklov problem involving the p(x)-Laplacian

Electronic Journal of Qualitative Theory of Differential Equations, 2014
Summary: This paper is concerned with the existence and multiplicity of solutions for \(p(x)\)-Laplacian Steklov problem without the well-known Ambrosetti-Rabinowitz type growth conditions. By means of critical point theorems with Cerami condition, under weaker conditions, existence and multiplicity results of the solutions are proved.
openaire   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Serum Metabolic Fingerprints Characterize Systemic Lupus Erythematosus

Advanced Science
Metabolic fingerprints in serum characterize diverse diseases for diagnostics and biomarker discovery. The identification of systemic lupus erythematosus (SLE) by serum metabolic fingerprints (SMFs) will facilitate precision medicine in SLE in an early ...
Shunxiang Li, Huihua Ding, Ziheng Qi, Jing Yang, Jingyi Huang, Lin Huang, Mengji Zhang, Yuanjia Tang, Nan Shen, Kun Qian, Qiang Guo, Jingjing Wan   +11 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Steklov problems involving the \(p(x)\)-Laplacian

Electronic Journal of Differential Equations, 2014
In this article, the authors study the problem \[ \Delta_{p(x)}u=a(x)|u|^{p(x)-2}u\text{, }x\in\Omega \] subject to the boundary condition \[ |\nabla u|^{p(x)-2}\frac{\partial u}{\partial\nu}=\lambda f(x,u)\text{, }x\in\partial\Omega. \] Here the set \(\Omega\subseteq\mathbb{R}^{N}\) is bounded with a smooth boundary, \(\lambda>0\) is a parameter, and \
Ghasem A. Afrouzi, Armin Hadjian, Shapour Heidarkhani   +2 more
openaire   +2 more sources

Estimating Derivatives in Nonseparable Models with Limited Dependent Variables [PDF]

We present a simple way to estimate the effects of changes in a vector of observable variables X on a limited dependent variable Y when Y is a general nonseparable function of X and unobservables.
Hidehiko Ichimura, Joseph G. Altonji, Taisuke Otsu   +2 more
core  

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source